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Con-FLIC：以单只果蝇分辨率同时测量进食行为和食物消耗。

Con-FLIC: concurrent measurement of feeding behaviors and food consumption in at single-fly resolution.

作者信息

Opoola Mubaraq, Fitzgerald Lucas, Hwangbo Dae-Sung

机构信息

Department of Biology, University of Louisville, Louisville, Kentucky, United States.

出版信息

MicroPubl Biol. 2024 Feb 28;2024. doi: 10.17912/micropub.biology.001128. eCollection 2024.

DOI:10.17912/micropub.biology.001128
PMID:38487478
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10938163/
Abstract

Accurate quantification of food intake and feeding behaviors are essential for understanding various biological processes, such as metabolism and aging. Currently, no methods allow for the concurrent measurement of both parameters for the same individual flies. Here, we couple Con-Ex ( Con sumption- Ex cretion) and FLIC ( F ly L iquid-Food I nteraction C ounter), previously developed to measure food consumption and various feeding behaviors, respectively, into a single platform that we named Con-FLIC. Using starvation as a known condition that changes food intake and feeding behaviors, we validate that Con-FLIC enables concurrent measurement of feeding behaviors and food intake in at a single-fly resolution. We expect that Con-FLIC will be an easy non-invasive option to quantify food consumption and feeding behaviors concurrently in the same individual flies.

摘要

准确量化食物摄入量和进食行为对于理解各种生物过程（如新陈代谢和衰老）至关重要。目前，尚无方法能够同时测量同一果蝇个体的这两个参数。在此，我们将先前分别用于测量食物消耗和各种进食行为的Con-Ex（消耗-排泄）和FLIC（果蝇液体食物相互作用计数器）结合到一个名为Con-FLIC的单一平台中。利用饥饿作为改变食物摄入量和进食行为的已知条件，我们验证了Con-FLIC能够以单果蝇分辨率同时测量进食行为和食物摄入量。我们期望Con-FLIC将成为一种简单的非侵入性方法，用于同时量化同一果蝇个体的食物消耗和进食行为。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b4e/10938163/a4bc8d4dbee4/25789430-2024-micropub.biology.001128.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b4e/10938163/a4bc8d4dbee4/25789430-2024-micropub.biology.001128.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b4e/10938163/a4bc8d4dbee4/25789430-2024-micropub.biology.001128.jpg

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Expansion and application of dye tracers for measuring solid food intake and food preference in Drosophila.染料示踪剂在测量果蝇固体食物摄入量和食物偏好中的扩展和应用。
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