Loqman Souad, Soraa Nabila, Diene Seydina M, Rolain Jean-Marc
Laboratoire de Lutte Contre les Maladies Infectieuses, Faculté de Médecine et de Pharmacie, Université Cadi Ayyad, Marrakech 40000, Morocco.
Laboratoire de Microbiologie, CHU Mohammed VI, Av Ibn Sina Amerchich, BP 2360, Marrakech 40000, Morocco.
Antibiotics (Basel). 2021 Apr 26;10(5):492. doi: 10.3390/antibiotics10050492.
The emergence and spread of carbapenem-resistant (CRE) represent a major clinical problem and raise serious health concerns. The present study aimed to investigate and ascertain the occurrence of CRE among hospitalized patients of Mohamed VI University Hospital, Marrakech, Morocco. Biological samples were collected over a one-year period (2018). The bacterial isolates were identified by MALDI-TOF-MS. Antibiotic susceptibility testing was performed using disc diffusion and Etest. The modified Hodge test and combined disc diffusion test were used for phenotypic detection. CRE hydrolyzing enzyme encoding genes: OXA-48, KPC, IMP, VIM, and NDM were characterized by PCR and DNA sequencing. In total, 131 non-duplicate CRE clinical strains resistant to Ertapenem were isolated out of 1603 initial . was the most common species (59%), followed by (24%), (10%), (3%), (2%), (1%), and (1%). Of these, 56.49%, 21.37%, 15.27%, 3.38%, and 3.05% were collected from blood, urine, pus, catheters and respiratory samples, respectively. Approximately 85.5% (112/131) of the isolates were carbapenemase producers (40 OXA-48, 27 NDM, 38 OXA-48 + NDM and 7 VIM). All metallo-β-lactamases isolates were NDM-1 and VIM-1 producers. This is the first documentation of OXA-48 genes from and in Morocco.
耐碳青霉烯类肠杆菌科细菌（CRE）的出现和传播是一个重大临床问题，并引发了严重的健康担忧。本研究旨在调查并确定摩洛哥马拉喀什穆罕默德六世大学医院住院患者中CRE的发生情况。在一年时间（2018年）内收集生物样本。通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）鉴定细菌分离株。使用纸片扩散法和Etest进行药敏试验。改良Hodge试验和联合纸片扩散试验用于表型检测。通过聚合酶链反应（PCR）和DNA测序对CRE水解酶编码基因：OXA-48、KPC、IMP、VIM和NDM进行特征分析。在1603株初始菌株中，共分离出131株对厄他培南耐药的非重复CRE临床菌株。大肠埃希菌是最常见的菌种（59%），其次是肺炎克雷伯菌（24%）、鲍曼不动杆菌（10%）、阴沟肠杆菌（3%）、产气肠杆菌（2%）、弗劳地枸橼酸杆菌（1%）和嗜麦芽窄食单胞菌（1%）。其中，分别从血液、尿液、脓液、导管和呼吸道样本中收集到的菌株占比为56.49%、21.37%、15.27%、3.38%和3.05%。大约85.5%（112/131）的分离株是碳青霉烯酶产生菌（40株OXA-48、27株NDM、38株OXA-48 + NDM和7株VIM）。所有金属β-内酰胺酶分离株均为NDM-1和VIM-1产生菌。这是摩洛哥首次记录来自大肠埃希菌和肺炎克雷伯菌的OXA-48基因。
