鲎试剂检测法、标准平板计数法和总大肠菌群计数法在再生废水微生物学评估中的比较。
Comparison of Limulus assay, standard plate count, and total coliform count for microbiological assessment of renovated wastewater.
作者信息
Jorgensen J H, Lee J C, Alexander G A, Wolf H W
出版信息
Appl Environ Microbiol. 1979 May;37(5):928-31. doi: 10.1128/aem.37.5.928-931.1979.
Abstract
The Limulus endotoxin assay was compared to the standard plate count and total coliform count for assessment of the bacteriological quality of reclaimed wastewater. A total of 48 water samples from an advanced waste treatment plant in Dallas, Tex. were examined by the three techniques. Limulus assays were technically simpler to perform and provided results much sooner than conventional culture methods. However, the endotoxin values did not correlate extremely well with determinations of viable bacterial numbers. This lack of correlation may have been due to alterations in the normal ratio of viable gram-negative cells to endotoxin caused by water reclamation procedures.
摘要
将鲎试剂内毒素测定法与标准平板计数法和总大肠菌群计数法进行比较，以评估再生废水的细菌学质量。对得克萨斯州达拉斯市一座高级废水处理厂的48个水样采用这三种技术进行了检测。鲎试剂测定法在技术上操作更简便，且比传统培养方法能更快得出结果。然而，内毒素值与活菌数的测定结果相关性不是非常好。这种缺乏相关性可能是由于水回收程序导致革兰氏阴性活菌与内毒素的正常比例发生了变化。
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