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鲎试剂检测法、标准平板计数法和总大肠菌群计数法在再生废水微生物学评估中的比较。

Comparison of Limulus assay, standard plate count, and total coliform count for microbiological assessment of renovated wastewater.

作者信息

Jorgensen J H, Lee J C, Alexander G A, Wolf H W

出版信息

Appl Environ Microbiol. 1979 May;37(5):928-31. doi: 10.1128/aem.37.5.928-931.1979.

DOI:10.1128/aem.37.5.928-931.1979
PMID:384901
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC243326/
Abstract

The Limulus endotoxin assay was compared to the standard plate count and total coliform count for assessment of the bacteriological quality of reclaimed wastewater. A total of 48 water samples from an advanced waste treatment plant in Dallas, Tex. were examined by the three techniques. Limulus assays were technically simpler to perform and provided results much sooner than conventional culture methods. However, the endotoxin values did not correlate extremely well with determinations of viable bacterial numbers. This lack of correlation may have been due to alterations in the normal ratio of viable gram-negative cells to endotoxin caused by water reclamation procedures.

摘要

将鲎试剂内毒素测定法与标准平板计数法和总大肠菌群计数法进行比较,以评估再生废水的细菌学质量。对得克萨斯州达拉斯市一座高级废水处理厂的48个水样采用这三种技术进行了检测。鲎试剂测定法在技术上操作更简便,且比传统培养方法能更快得出结果。然而,内毒素值与活菌数的测定结果相关性不是非常好。这种缺乏相关性可能是由于水回收程序导致革兰氏阴性活菌与内毒素的正常比例发生了变化。

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本文引用的文献

1
Rapid determination of bacteriological water quality by using limulus lysate.利用鲎试剂快速测定水质的细菌学质量。
Appl Environ Microbiol. 1978 Feb;35(2):376-82. doi: 10.1128/aem.35.2.376-382.1978.
2
Radiometric method for the detection of coliform organisms in water.水中大肠菌群生物检测的辐射测量法。
Appl Microbiol. 1974 Aug;28(2):169-71. doi: 10.1128/am.28.2.169-171.1974.
3
Rapid detection of contaminated intravenous fluids using the Limulus in vitro endotoxin assay.利用鲎试剂体外内毒素测定法快速检测受污染的静脉输液。
Appl Microbiol. 1973 Oct;26(4):521-4. doi: 10.1128/am.26.4.521-524.1973.
4
Rapid detection of gram-negative bacteriuria by use of the Limulus endotoxin assay.利用鲎试剂内毒素测定法快速检测革兰氏阴性菌尿症
Appl Microbiol. 1973 Jul;26(1):38-42. doi: 10.1128/am.26.1.38-42.1973.
5
Measurement of bound and free endotoxin by the Limulus assay.用鲎试剂法测定结合内毒素和游离内毒素。
Proc Soc Exp Biol Med. 1974 Sep;146(4):1024-31. doi: 10.3181/00379727-146-38240.
6
Preparation, sensitivity, and specificity of Limulus lysate for endotoxin assay.鲎试剂用于内毒素检测的制备、灵敏度和特异性。
Appl Microbiol. 1973 Jul;26(1):43-8. doi: 10.1128/am.26.1.43-48.1973.
7
Bacteriophages and endotoxin in licensed live-virus vaccines.
J Infect Dis. 1975 May;131(5):588-91. doi: 10.1093/infdis/131.5.588.
8
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