Fliermans C B, Cherry W B, Orrison L H, Thacker L
Appl Environ Microbiol. 1979 Jun;37(6):1239-42. doi: 10.1128/aem.37.6.1239-1242.1979.
Continuous centrifugation of large volumes of water from natural southeastern lakes allowed quantitative detection of Legionella pneumophila by direct immunofluorescent staining. Positive samples were injected intraperitoneally into guinea pigs, and the L. pneumophila were isolated and identified by their morphological, cultural, physiological, and serological characteristics.
对来自东南部天然湖泊的大量水样进行连续离心,通过直接免疫荧光染色可对嗜肺军团菌进行定量检测。将阳性样本腹腔注射到豚鼠体内,然后根据嗜肺军团菌的形态、培养、生理和血清学特征对其进行分离和鉴定。