Department of Biology, Faculty of Science, Hacettepe University, Ankara, Turkey.
Department of Physics Engineering, Faculty of Engineering, Hacettepe University, Ankara, Turkey.
Biopolymers. 2024 May;115(3):e23572. doi: 10.1002/bip.23572. Epub 2024 Mar 16.
As a natural and biocompatible material with high strength and flexibility, spider silk is frequently used in biomedical studies. In this study, the availability of Argiope bruennichi spider silk as a surgical suture material was investigated. The effects of spider silk-based and commercial sutures, with and without Aloe vera coating, on wound healing were evaluated by a rat dorsal skin flap model, postoperatively (7th and 14th days). Biochemical, hematological, histological, immunohistochemical, small angle x-ray scattering (SAXS) analyses and mechanical tests were performed. A. bruennichi silk did not show any cytotoxic effect on the L929 cell line according to MTT and LDH assays, in vitro. The silk materials did not cause any allergic reaction, infection, or systemic effect in rats according to hematological and biochemical analyses. A. bruennichi spider silk group showed a similar healing response to commercial sutures. SAXS analysis showed that the 14th-day applications of A. bruennichi spider silk and A. vera coated commercial suture groups have comparable structural results with control group. In conclusion, A. bruennichi spider silk is biocompatible in line with the parameters examined and shows a healing response similar to the commercial sutures commonly used in the skin.
作为一种具有高强度和柔韧性的天然生物相容性材料，蜘蛛丝经常被用于生物医学研究。本研究旨在探讨赤纺足蛛丝作为外科缝线的可行性。通过大鼠背部皮瓣模型，评估了未涂覆和涂覆有芦荟的基于蜘蛛丝的缝线和市售缝线对伤口愈合的影响，术后第 7 天和第 14 天进行了评估。进行了生物化学、血液学、组织学、免疫组织化学、小角 X 射线散射（SAXS）分析和力学测试。MTT 和 LDH 测定体外实验表明，赤纺足蛛丝对 L929 细胞系没有任何细胞毒性作用。根据血液学和生化学分析，蛛丝材料在大鼠中未引起任何过敏反应、感染或全身作用。赤纺足蛛丝组的愈合反应与市售缝线相似。SAXS 分析表明，第 14 天应用赤纺足蛛丝和涂覆有芦荟的市售缝线组与对照组具有相似的结构结果。总之，赤纺足蛛丝在检查的参数范围内具有生物相容性，并且其愈合反应与皮肤中常用的市售缝线相似。
