College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350108, PR China.
Burns. 2010 Sep;36(6):891-6. doi: 10.1016/j.burns.2009.12.001. Epub 2010 Feb 18.
This study investigates the feasibility of recombinant spider silk protein as a wound-dressing material for coverage of deep second-degree burn wounds using an animal model. Sixty Sprague-Dawley (SD) rats were randomly divided into four groups (15 rats in each group). Two types of recombinant spider silk proteins, pNSR-16 and pNSR-32, as well as collagen (as a control) were applied on the wound; the fourth group was left untreated as a negative control. Each group was evaluated on the 3rd, 5th, 7th, 14th and 21st days for wound-healing rate, histological test, levels of hydroxyproline synthesis and the samples were stained for immunohistochemical detection of the basic fibroblast growth factor (bFGF). The results of implantation testing showed that wound healing in the treatment groups--recombinant spider silk protein pNSR-16 and pNSR-32--was much better than that in the control group (p<0.01). On the 7th, 14th and 21st days, higher expression of bFGF and the increase of hydroxyproline content of the skin indicated good regeneration of wound skin in the treatment groups. Preliminarily, we conclude that the recombinant spider silk protein membrane promotes the recovery of wound skin by increasing the expression and secretion of the growth factor bFGF and hydroxyproline.
本研究通过动物模型探讨了重组蜘蛛丝蛋白作为深度二度烧伤创面覆盖物的可行性。60 只 Sprague-Dawley（SD）大鼠随机分为四组（每组 15 只）。两种类型的重组蜘蛛丝蛋白，pNSR-16 和 pNSR-32，以及胶原蛋白（作为对照）应用于伤口；第四组作为阴性对照不做任何处理。每组在第 3、5、7、14 和 21 天进行伤口愈合率、组织学测试、羟脯氨酸合成水平评估，并对样本进行碱性成纤维细胞生长因子（bFGF）的免疫组织化学检测染色。植入试验结果表明，治疗组-重组蜘蛛丝蛋白 pNSR-16 和 pNSR-32 的伤口愈合情况明显优于对照组（p<0.01）。在第 7、14 和 21 天，bFGF 的高表达和皮肤中羟脯氨酸含量的增加表明治疗组的伤口皮肤有良好的再生。初步得出结论，重组蜘蛛丝蛋白膜通过增加生长因子 bFGF 和羟脯氨酸的表达和分泌来促进伤口皮肤的恢复。
