Kiselev Konstantin V, Suprun Andrey R, Aleynova Olga A, Ogneva Zlata V, Dubrovina Alexandra S
Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Russia.
Plants (Basel). 2024 Feb 16;13(4):541. doi: 10.3390/plants13040541.
Plant surface treatment with double-stranded RNAs (dsRNAs) has gained recognition as a promising method for inducing gene silencing and combating plant pathogens. However, the regulation of endogenous plant genes by external dsRNAs has not been sufficiently investigated. Also, the effect of the simultaneous application of multiple gene-specific dsRNAs has not been analyzed. The aim of this study was to exogenously target five genes in , namely, three transcription factor genes (, , ), a calmodulin-binding protein gene (), and an anthocyanidin reductase gene (), which are known as negative regulators of anthocyanin accumulation. Exogenous dsRNAs encoding these genes were applied to the leaf surface of either individually or in mixtures. The mRNA levels of the five targets were analyzed using qRT-PCR, and anthocyanin content was evaluated through HPLC-MS. The results demonstrated significant downregulation of all five target genes by the exogenous dsRNAs, resulting in enhanced expression of chalcone synthase () gene and increased anthocyanin content. The simultaneous foliar application of the five dsRNAs proved to be more efficient in activating anthocyanin accumulation compared to the application of individual dsRNAs. These findings hold considerable importance in plant biotechnology and gene function studies.
用双链RNA（dsRNAs）进行植物表面处理已成为一种诱导基因沉默和对抗植物病原体的有前景的方法。然而，外源dsRNAs对植物内源基因的调控尚未得到充分研究。此外，同时应用多种基因特异性dsRNAs的效果也未进行分析。本研究的目的是外源靶向[植物名称未给出]中的五个基因，即三个转录因子基因（[基因名称未给出]、[基因名称未给出]、[基因名称未给出]）、一个钙调蛋白结合蛋白基因（[基因名称未给出]）和一个花青素还原酶基因（[基因名称未给出]），这些基因被认为是花青素积累的负调控因子。编码这些基因的外源dsRNAs单独或混合施用于[植物名称未给出]的叶片表面。使用qRT-PCR分析五个靶标的mRNA水平，并通过HPLC-MS评估花青素含量。结果表明，外源dsRNAs显著下调了所有五个靶基因，导致查尔酮合酶（[基因名称未给出]）基因表达增强和花青素含量增加。与单独施用dsRNAs相比，同时叶面施用五种dsRNAs在激活花青素积累方面更有效。这些发现在植物生物技术和基因功能研究中具有相当重要的意义。
