Zhao Yujin, Zhou Yanguang, Xu Jingyan, Fan Sen, Zhu Na, Meng Qingling, Dai Shijie, Yuan Xiaofeng
School of Life Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Plants (Basel). 2024 Sep 27;13(19):2712. doi: 10.3390/plants13192712.
RNA interference (RNAi) shows great potential in plant defense against pathogens through RNA-mediated sequence-specific gene silencing. Among RNAi-based plant protection strategies, spray-induced gene silencing (SIGS) is considered a more promising approach because it utilizes the transfer of exogenous RNA between plants and microbes to silence target pathogen genes. The application of nanovesicles significantly enhances RNA stability and delivery efficiency, thereby improving the effectiveness of SIGS and further enhancing plant resistance to diseases and pathogens. This review explores the role of RNAi in plant protection, focusing on the cross-kingdom transport of small RNAs (sRNAs) via extracellular vesicles. It also explores the potential of nanotechnology to further optimize RNA-based plant protection, offering innovative tools and methods in modern plant biotechnology.
RNA干扰（RNAi）通过RNA介导的序列特异性基因沉默在植物抵御病原体方面显示出巨大潜力。在基于RNAi的植物保护策略中，喷雾诱导基因沉默（SIGS）被认为是一种更具前景的方法，因为它利用植物与微生物之间的外源RNA转移来沉默目标病原体基因。纳米囊泡的应用显著提高了RNA的稳定性和递送效率，从而提高了SIGS的有效性，并进一步增强了植物对疾病和病原体的抗性。本综述探讨了RNAi在植物保护中的作用，重点关注小RNA（sRNA）通过细胞外囊泡的跨界运输。它还探讨了纳米技术进一步优化基于RNA的植物保护的潜力，为现代植物生物技术提供创新的工具和方法。
