RNA 干扰：在植物中的过去和未来应用。
RNA Interference Past and Future Applications in Plants.
机构信息
Department of Plant Agriculture, University of Guelph, 50 Stone Road E., Guelph, ON N1G 2W1, Canada.
Research Centre, Agriculture and Agri-Food Canada, 5403 1 Ave S., Lethbridge, AB T1J 4B1, Canada.
出版信息
Int J Mol Sci. 2023 Jun 5;24(11):9755. doi: 10.3390/ijms24119755.
Antisense RNA was observed to elicit plant disease resistance and post-translational gene silencing (PTGS). The universal mechanism of RNA interference (RNAi) was shown to be induced by double-stranded RNA (dsRNA), an intermediate produced during virus replication. Plant viruses with a single-stranded positive-sense RNA genome have been instrumental in the discovery and characterization of systemic RNA silencing and suppression. An increasing number of applications for RNA silencing have emerged involving the exogenous application of dsRNA through spray-induced gene silencing (SIGS) that provides specificity and environmentally friendly options for crop protection and improvement.
反义 RNA 被观察到能引发植物的疾病抗性和翻译后基因沉默（PTGS）。双链 RNA（dsRNA）诱导的 RNA 干扰（RNAi）的普遍机制被证明是由病毒复制过程中产生的一种中间产物引起的。具有单链正链 RNA 基因组的植物病毒在发现和描述系统性 RNA 沉默和抑制方面发挥了重要作用。越来越多的 RNA 沉默应用通过喷雾诱导基因沉默（SIGS）出现，该技术通过外源应用 dsRNA 提供了作物保护和改良的特异性和环保选择。
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