Department of Clinical Laboratory, The Affiliated Taian City Central Hospital of Qingdao University, Taian, 271000, China.
Shandong Provincial Key Medical and Health Laboratory of Anti-drug Resistant Drug Research, Taian City Central Hospital, Taian, 271000, China.
Clin Transl Oncol. 2024 Aug;26(8):1988-1997. doi: 10.1007/s12094-024-03423-6. Epub 2024 Mar 19.
tRF-RNA-a representative of non-coding RNA (ncRNA)-is a precursor or fragment of mature tRNA and plays a crucial regulatory role in the occurrence and development of cancer. There is currently little research on tRF-RNA as a diagnostic marker in cancer, especially for NSCLC from serum exosomes.
Serum exosomes were successfully extracted from serum; their physical morphology was captured by transmission electron microscopy (TEM); appropriate particle size detection was performed using qNano; surface labeling was verified through western blotting. Serum exosomes i-tRF-AspGTC and tRF-1-SerCGA were selected through gene microarray, and qPCR was used to validate their significance in 242 patients and 201 healthy individuals. The area under the curve (AUC) was used to evaluate the diagnostic indicators of non-small cell lung cancer (NSCLC).
Compared with 201 healthy individuals, i-tRF-AspGTC and tRF-1-SerCGA were significantly downregulated in 242 NSCLC patients and 95 early-stage patients. For tRF-AspGTC and tRF-1-SerCGA, the predictive diagnostic efficiency rates of AUC were 0.690 and 0.680, respectively, whereas the early diagnostic efficiency rates were 0.656 and 0.688, respectively. The result of combined diagnosis with CEA and CYFRA21-1 was 0.928, and the early diagnostic efficiency was 0.843, which is a very high biological predictive factor for NSCLC.
The expression of serum exosomes i-tRF-AspGTC and tRF-1-SerCGA was significantly downregulated in NSCLC patients. These exosomes could be used as predictive indicators for diagnosis or early diagnosis of NSCLC.
tRF-RNA-非编码 RNA (ncRNA) 的代表-是成熟 tRNA 的前体或片段，在癌症的发生和发展中发挥着关键的调节作用。目前，关于 tRF-RNA 作为癌症诊断标志物的研究较少，特别是针对血清外泌体中的非小细胞肺癌 (NSCLC)。
成功从血清中提取血清外泌体；通过透射电子显微镜 (TEM) 捕获其物理形态；使用 qNano 进行适当的颗粒大小检测；通过 Western blot 验证表面标记。通过基因微阵列选择血清外泌体 i-tRF-AspGTC 和 tRF-1-SerCGA，并用 qPCR 验证它们在 242 例患者和 201 例健康个体中的意义。曲线下面积 (AUC) 用于评估非小细胞肺癌 (NSCLC) 的诊断指标。
与 201 例健康个体相比，242 例 NSCLC 患者和 95 例早期患者的 i-tRF-AspGTC 和 tRF-1-SerCGA 明显下调。对于 tRF-AspGTC 和 tRF-1-SerCGA，AUC 的预测诊断效率分别为 0.690 和 0.680，而早期诊断效率分别为 0.656 和 0.688。CEA 和 CYFRA21-1 联合诊断的结果为 0.928，早期诊断效率为 0.843，这是 NSCLC 的一个非常高的生物预测因子。
NSCLC 患者血清外泌体 i-tRF-AspGTC 和 tRF-1-SerCGA 的表达明显下调。这些外泌体可作为 NSCLC 诊断或早期诊断的预测指标。
