Joho M, Matsumoto H, Tohoyama H, Murayama T
Biochim Biophys Acta. 1979 Jul 4;585(3):383-8. doi: 10.1016/0304-4165(79)90082-5.
The activity of dehydrogenase in Saccharomyces cerevisiae was estimated by reduction of 2,3,5-triphenyltetrazolium chloride. By the adaptation of yeast to cadmium, the high activity of dehydrogenase was observed. Furthermore, the activity of dehydrogenase in Cd-resistant cells was increased by growing in medium containing CdSO4. However, the activity of dehydrogenase was inhibited by the addition of CdSO4 to the reaction mixture. The activity of dehydrogenase in Cd-sensitive cells was increased slightly by incubation with low concentrations of CdSO4. High activity of dehydrogenase in Cd-resistant cells was completely negated by the addition of cycloheximide to the incubation medium. The increase of dehydrogenase activity is due partly to de novo synthesis of protein.
通过2,3,5-氯化三苯基四氮唑的还原反应来估算酿酒酵母中脱氢酶的活性。通过使酵母适应镉，观察到脱氢酶的高活性。此外，抗镉细胞中的脱氢酶活性通过在含有硫酸镉的培养基中生长而增加。然而，向反应混合物中添加硫酸镉会抑制脱氢酶的活性。低浓度硫酸镉孵育会使镉敏感细胞中的脱氢酶活性略有增加。向孵育培养基中添加环己酰亚胺可完全消除抗镉细胞中脱氢酶的高活性。脱氢酶活性的增加部分归因于蛋白质的从头合成。
