Zeynep Kamil Gynecology and Obstetrics Training and Research Hospital, İstanbul, Turkey.
Corresponding author: Dr Eralp Bulutlar, Başakşehir Çam and Sakura City Hospital, Başakşehir Olimpiyat Bulvarı Yolu, 34480 Başakşehir, İstanbul, Turkey,
Diving Hyperb Med. 2024 Mar 31;54(1):16-22. doi: 10.28920/dhm54.1.16-22.
This study aimed to investigate whether hyperbaric oxygen treatment (HBOT) could ameliorate ischaemia-reperfusion injury in a rat model of ovarian torsion-detorsion.
Twenty-seven rats were divided among four groups: surgical sham rats (S) (n = 6) underwent identical anaesthesia and surgical incisions to other groups (n = 7 per group) but with no ovary intervention; torsion rats (T) underwent laparotomy, ovarian torsion, relaparotomy and sacrifice after three hours; torsion and detorsion rats (T/DT) underwent laparotomy, ovarian torsion (three hours), relaparotomy and detorsion, and sacrifice after one week; torsion, detorsion, hyperbaric oxygen rats (T/DT/HBOT) underwent laparotomy, ovarian torsion, relaparotomy and detorsion, and sacrifice after one week during which HBOT was provided 21 times (100% oxygen at 600 kPa for 50 min). In all groups blood collection for markers of oxidative stress or related responses, and ovary collection for histology were performed after sacrifice.
When the T/DT, and T/DT/HBOT groups were compared, 8-hydroxy-2'-deoxyguanosine (a marker of oxidative damage to DNA) and malondialdehyde (a product of lipid peroxidation) levels were lower in the T/DT/HBOT group. Anti-Mullerian hormone levels were higher in the T/DT/HBOT group compared to the T/DT group. In addition, oedema, vascular occlusion, neutrophilic infiltration and follicular cell damage were less in the T/DT/HBOT group than in the T/DT group.
When biochemical and histopathological findings were evaluated together, HBOT appeared reduce ovarian ischaemia / reperfusion injury in this rat model of ovarian torsion-detorsion.
本研究旨在探讨高压氧治疗（HBOT）是否可以改善卵巢扭转复位大鼠模型中的缺血再灌注损伤。
27 只大鼠分为四组：手术假手术大鼠（S）（n=6）接受与其他组相同的麻醉和手术切口，但卵巢无干预（n=7/组）；扭转组（T）行剖腹术、卵巢扭转、再剖腹术和 3 小时后处死；扭转复位组（T/DT）行剖腹术、卵巢扭转（3 小时）、再剖腹术和复位，1 周后处死；扭转复位、高压氧组（T/DT/HBOT）行剖腹术、卵巢扭转、再剖腹术和复位，1 周后行 HBOT（600kPa 下 100%氧气 50min）21 次，各组均在处死前采集血样检测氧化应激标志物或相关反应标志物，采集卵巢组织行组织学检查。
与 T/DT 组相比，T/DT/HBOT 组 8-羟基-2'-脱氧鸟苷（DNA 氧化损伤标志物）和丙二醛（脂质过氧化产物）水平较低，T/DT/HBOT 组抗苗勒管激素水平较高。与 T/DT 组相比，T/DT/HBOT 组卵巢水肿、血管闭塞、中性粒细胞浸润和卵泡细胞损伤较少。
综合生化和组织病理学结果，HBOT 似乎可以减轻该卵巢扭转复位大鼠模型中的卵巢缺血再灌注损伤。
