Department of Cell and Molecular Pharmacology & Experimental Therapeutics, Medical University of South Carolina, Charleston, SC, USA.
FEBS Lett. 2024 Mar;598(6):602-620. doi: 10.1002/1873-3468.14852. Epub 2024 Mar 21.
The extracellular matrix (ECM) proteome represents an important component of the tissue microenvironment that controls chemical flux and induces cell signaling through encoded structure. The analysis of the ECM represents an analytical challenge through high levels of post-translational modifications, protease-resistant structures, and crosslinked, insoluble proteins. This review provides a comprehensive overview of the analytical challenges involved in addressing the complexities of spatially profiling the extracellular matrix proteome. A synopsis of the process of synthesizing the ECM structure, detailing inherent chemical complexity, is included to present the scope of the analytical challenge. Current chromatographic and spatial techniques addressing these challenges are detailed. Capabilities for multimodal multiplexing with cellular populations are discussed with a perspective on developing a holistic view of disease processes that includes both the cellular and extracellular microenvironment.
细胞外基质（ECM）蛋白质组代表了组织微环境的一个重要组成部分，通过编码结构控制化学通量并诱导细胞信号转导。ECM 的分析是一个分析挑战，因为它存在高水平的翻译后修饰、蛋白酶抗性结构和交联的不溶性蛋白质。本综述全面概述了在解决细胞外基质蛋白质组空间分析复杂性方面所涉及的分析挑战。概述了合成 ECM 结构的过程，详细介绍了内在的化学复杂性，以呈现分析挑战的范围。详细介绍了当前用于解决这些挑战的色谱和空间技术。讨论了与细胞群体进行多模式多重分析的能力，并从包括细胞和细胞外微环境在内的整体疾病过程的角度展望了未来的发展。
