Department of Biophysics, Molecular Biology and Bioinformatics, University of Calcutta, 92, A.P.C. Road, Kolkata - 700009, West Bengal, India.
Department of Biophysics, Molecular Biology and Bioinformatics, University of Calcutta, 92, A.P.C. Road, Kolkata - 700009, West Bengal, India.
Plant Physiol Biochem. 2024 Apr;209:108533. doi: 10.1016/j.plaphy.2024.108533. Epub 2024 Mar 19.
Selenium (Se) toxicity is an emerging contaminant of global concern. It is known to cause oxidative stress, affecting plant growth and yield. Plantago ovata, a major cash crop known for its medicinal properties, is often cultivated in Se-contaminated soil. Thus, the aim of this study was to evaluate the use of methyl jasmonate (MeJA) seed priming technique to mitigate Se-induced phytotoxicity. The results demonstrated that Se stress inhibited P. ovata growth, biomass and lowered chlorophyll content in a dose-dependent manner. Treatment with 1 μM MeJA enhanced the antioxidant defence system via ROS signalling and upregulated key enzymes of phenylpropanoid pathway, PAL (1.9 times) and CHI (5.4 times) in comparison to control. Caffeic acid, Vanillic acid, Chlorogenic acid, Coumaric acid and Luteoloside were the most abundant polyphenols. Enzymatic antioxidants involved in ROS scavenging, such as CAT (up to 1.3 times) and GPOX (up to 1.4 times) were raised, while SOD (by 0.6 times) was reduced. There was an upregulation of growth-inducible hormones, IAA (up to 2.1 fold) and GA (up to 1.5 fold) whereas, the stress-responsive hormones ABA (by 0.6 fold) and SA (by 0.5 fold) were downregulated. The alleviation of Se toxicity was also evident from the decrease in HO and MDA contents under MeJA treatment. These findings suggest that MeJA can effectively improve Se tolerance and nutraceutical value in P. ovata by modulating the phytohormone regulatory network, redox homeostasis and elicits accumulation of polyphenols. Therefore, MeJA seed priming could be an efficient way to enhance stress resilience and sustainable crop production.
硒（Se）毒性是一种全球关注的新兴污染物。它已知会导致氧化应激，影响植物的生长和产量。车前草是一种主要的经济作物，以其药用特性而闻名，常种植在受硒污染的土壤中。因此，本研究旨在评估茉莉酸甲酯（MeJA）种子引发技术在减轻硒诱导的植物毒性方面的应用。结果表明，硒胁迫以剂量依赖的方式抑制了车前草的生长、生物量和降低了叶绿素含量。与对照相比，用 1 μM MeJA 处理通过 ROS 信号增强了抗氧化防御系统，并上调了苯丙烷途径的关键酶 PAL（1.9 倍）和 CHI（5.4 倍）。咖啡酸、香草酸、绿原酸、香豆酸和芦丁是最丰富的多酚。参与 ROS 清除的酶促抗氧化剂，如 CAT（高达 1.3 倍）和 GPOX（高达 1.4 倍）增加，而 SOD（减少 0.6 倍）。生长诱导激素 IAA（高达 2.1 倍）和 GA（高达 1.5 倍）上调，而应激响应激素 ABA（减少 0.6 倍）和 SA（减少 0.5 倍）下调。MeJA 处理后 HO 和 MDA 含量的降低也表明硒毒性得到缓解。这些发现表明，MeJA 可以通过调节植物激素调控网络、氧化还原平衡和诱导多酚积累，有效地提高车前草的硒耐受性和营养品质。因此，MeJA 种子引发可能是增强植物抗逆性和可持续作物生产的有效途径。
