Engineering Research Center of Western Resource Innovation Medicine Green Manufacturing, Ministry of Education, School of Chemical Engineering, Northwest University, Xi'an 710069, China.
Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710069, China.
J Agric Food Chem. 2024 Apr 3;72(13):7266-7278. doi: 10.1021/acs.jafc.3c09158. Epub 2024 Mar 24.
Ginseng oligopeptides are naturally occurring small-molecule peptides extracted from ginseng that exhibit positive effects on health and longevity. However, the current industrial production of ginseng oligopeptides primarily relies on plant extraction and chemical synthesis. In this study, we proposed a novel genetic engineering approach to produce active ginseng peptides through multicopy tandem insertion (5 and 15 times). The recombinant ginseng peptides were successfully produced from engineered with an increasing yield from 356.55 to 2900 mg/L as the repeats multiple. Additionally, an oxidative stress-induced aging model caused by HO was established to evaluate whether the recombinant ginseng peptides, without enzymatic hydrolysis into individual peptides, also have positive effects on antiaging. The results demonstrated that all two kinds of recombinant ginseng peptides could also delay cellular aging through various mechanisms, such as inhibiting cell cycle arrest, suppressing the expression of pro-inflammatory factors, and enhancing cellular antioxidant capacity.
人参寡肽是从人参中提取的天然小分子肽，对健康和长寿有积极作用。然而，目前人参寡肽的工业生产主要依赖于植物提取和化学合成。本研究提出了一种通过多拷贝串联插入（5 倍和 15 倍）生产活性人参肽的新的遗传工程方法。通过工程菌成功生产了重组人参肽，随着重复次数的增加，产量从 356.55 增加到 2900mg/L。此外，还建立了由 HO 引起的氧化应激诱导的衰老模型，以评估重组人参肽在未经酶解成单个肽的情况下是否也具有抗衰老作用。结果表明，这两种重组人参肽都可以通过多种机制延缓细胞衰老，如抑制细胞周期停滞、抑制促炎因子的表达和增强细胞抗氧化能力。
