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噬菌体制备及其对. 生物膜抑制效果的表征。

Characterization of a  phage vB_AbaM-SHI and its inhibition effect on biofilms of .

机构信息

School of Basic Medical Sciences, Health Science Center, Ningbo University, Ningbo, Zhejiang, China.

Department of Blood Transfusion, Hubei No. 3 People's Hospital of Jianghan University, Wuhan, Hubei, China.

出版信息

Front Cell Infect Microbiol. 2024 Mar 8;14:1351993. doi: 10.3389/fcimb.2024.1351993. eCollection 2024.

DOI:10.3389/fcimb.2024.1351993
PMID:38524182
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10958429/
Abstract

() is a popular clinical pathogen worldwide. Biofilm-associated antibiotic-resistant infection poses a great threat to human health. Bacteria in biofilms are highly resistant to antibiotics and disinfectants. Furthermore, inhibition or eradication of biofilms in husbandry, the food industry and clinics are almost impossible. Phages can move across the biofilm matrix and promote antibiotic penetration. In the present study, a lytic phage vB_AbaM-SHI, belonging to family , was isolated from sauce chop factory drain outlet in Wuxi, China. The DNA genome consists of 44,180 bp which contain 93 open reading frames, and genes encoding products morphogenesis are located at the end of the genome. The amino acid sequence of vB_AbaM-SHI endolysin is different from those of previously reported phages in NCBI. Phage vB_AbaM-SHI endolysin has two additional β strands due to the replacement of a lysine (K) (in KU510289.1, NC_041857.1, JX976549.1 and MH853786.1) with an arginine (R) (SHI) at position 21 of phage endolysin. Spot test showed that phage vB_AbaM-SHI is able to lyse some antibiotic-resistant bacteria, such as (SL, SL1, and SG strains) and BL21 strain. Additionally, phage vB_AbaM-SHI independently killed bacteria and inhibited bacterial biofilm formation, and synergistically exerted strong antibacterial effects with antibiotics. This study provided a new perspective into the potential application value of phage vB_AbaM-SHI as an antimicrobial agent.

摘要

（）是一种全球流行的临床病原体。生物膜相关的抗生素耐药性感染对人类健康构成了巨大威胁。生物膜中的细菌对抗生素和消毒剂具有高度抗性。此外，在畜牧业、食品工业和临床中几乎不可能抑制或消除生物膜。噬菌体可以穿过生物膜基质并促进抗生素渗透。本研究从中国无锡酱油厂排水口分离到一株裂解性噬菌体 vB_AbaM-SHI，属于科。其 DNA 基因组由 44180bp 组成，包含 93 个开放阅读框，编码形态发生的基因位于基因组末端。vB_AbaM-SHI 噬菌体溶菌酶的氨基酸序列与 NCBI 中先前报道的噬菌体不同。噬菌体 vB_AbaM-SHI 溶菌酶由于在位置 21 用精氨酸（R）（在 SHI 中）取代赖氨酸（K）（在 KU510289.1、NC_041857.1、JX976549.1 和 MH853786.1 中），导致其具有两个额外的β链。点试验表明，噬菌体 vB_AbaM-SHI 能够裂解一些抗生素耐药菌，如（SL、SL1 和 SG 株）和 BL21 菌株。此外，噬菌体 vB_AbaM-SHI 能够独立杀死细菌并抑制细菌生物膜形成，与抗生素协同发挥强大的抗菌作用。本研究为噬菌体 vB_AbaM-SHI 作为抗菌剂的潜在应用价值提供了新的视角。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3590/10958429/a0bcc859929c/fcimb-14-1351993-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3590/10958429/4e820336b3e2/fcimb-14-1351993-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3590/10958429/c83108de8a54/fcimb-14-1351993-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3590/10958429/e6535f0f9a47/fcimb-14-1351993-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3590/10958429/29c8324176c2/fcimb-14-1351993-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3590/10958429/696d318333c0/fcimb-14-1351993-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3590/10958429/e5546d233c90/fcimb-14-1351993-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3590/10958429/d1b262461755/fcimb-14-1351993-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3590/10958429/da3abcc12763/fcimb-14-1351993-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3590/10958429/a0bcc859929c/fcimb-14-1351993-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3590/10958429/4e820336b3e2/fcimb-14-1351993-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3590/10958429/c83108de8a54/fcimb-14-1351993-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3590/10958429/e6535f0f9a47/fcimb-14-1351993-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3590/10958429/29c8324176c2/fcimb-14-1351993-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3590/10958429/696d318333c0/fcimb-14-1351993-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3590/10958429/e5546d233c90/fcimb-14-1351993-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3590/10958429/d1b262461755/fcimb-14-1351993-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3590/10958429/da3abcc12763/fcimb-14-1351993-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3590/10958429/a0bcc859929c/fcimb-14-1351993-g009.jpg

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