State Key Laboratory for Quality and Safety of Agroproducts, Ningbo University, Ningbo, China.
Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo University, Ningbo, China.
PLoS One. 2023 Sep 1;18(9):e0289895. doi: 10.1371/journal.pone.0289895. eCollection 2023.
Vibrio splendidus is a common pathogen in the ocean that infects Apostichopus japonicus, Atlantic salmon and Crassostrea gigas, leading to a variety of diseases. In this study, a virulent phage vB_VspM_VS1, which infects V. splendidus, was isolated from aquaculture ponds in Dalian, China, and it belongs to the family Straboviridae in the order Caudoviricetes. vB_VspM_VS1 had an adsorption rate of 96% in 15 min, a latent period of 65 min, and a burst size of 140 ± 6 PFU/cell. The complete genome of phage vB_VspM_VS1 consists of a linear double-stranded DNA that is 248,270 bp in length with an average G + C content of 42.5% and 389 putative protein-coding genes; 116 genes have known functions. There are 4 tail fiber genes in the positive and negative strands of the phage vB_VspM_VS1 genome. The protein domain of the phage vB_VspM_VS1 tail fibers was obtained from the Protein Data Bank and the SMART (http://smart.embl.de) database. Bacterial challenge tests revealed that the growth of V. splendidus HS0 was apparently inhibited (OD600 < 0.01) in 12 h at an MOI of 10. In against biofilms, we also showed that the OD570 value of the vB_VspM_VS1-treated group (MOI = 1) decreased significantly to 0.04 ± 0.01 compared with that of the control group (0.48 ± 0.08) at 24 h. This study characterizes the genome of the phage vB_VspM_VS1 that infects the pathogenic bacterium V. splendidus of A. japonicus.
灿烂弧菌是一种常见的海洋病原体，可感染刺参、大西洋鲑和牡蛎，导致多种疾病。本研究从中国大连的水产养殖池塘中分离到一株感染灿烂弧菌的烈性噬菌体 vB_VspM_VS1，属于长尾病毒目 Straboviridae 科。vB_VspM_VS1 在 15 分钟内的吸附率为 96%，潜伏期为 65 分钟，爆发量为 140±6PFU/细胞。噬菌体 vB_VspM_VS1 的全基因组为线性双链 DNA，长度为 248270bp，平均 G+C 含量为 42.5%，有 389 个推定的蛋白编码基因；其中 116 个基因具有已知功能。噬菌体 vB_VspM_VS1 的基因组在正、负链上各有 4 个尾丝基因。噬菌体 vB_VspM_VS1 尾丝蛋白的结构域从蛋白质数据库和 SMART（http://smart.embl.de）数据库中获得。细菌攻毒试验表明，当感染复数（MOI）为 10 时，噬菌体 vB_VspM_VS1 在 12 小时内可明显抑制灿烂弧菌 HS0 的生长（OD600<0.01）。在抗生物膜方面，我们还表明，与对照组（0.48±0.08）相比，vB_VspM_VS1 处理组（MOI=1）的 OD570 值在 24 小时时显著下降至 0.04±0.01。本研究对感染刺参致病性细菌灿烂弧菌的噬菌体 vB_VspM_VS1 的基因组进行了特征描述。
