Hasnain Ali, Dadkhah-Aghdash Hamed, Luqman Muhammad, Muhammad Sohaib, Sardar Andleeb Anwar, Ali Shaukat, Mehmood Farhat, Khan Usman Ahmed, Mehmood Zahid, John Arooba, Khan Zafar Iqbal, Yang Hsi-Hsien, Farooq Awan Muhammad Umer
Department of Botany, Government College University, Lahore, 54000, Pakistan.
Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
Heliyon. 2024 Mar 14;10(6):e28157. doi: 10.1016/j.heliyon.2024.e28157. eCollection 2024 Mar 30.
Automobile exhaust releases different types of pollutants that are at great risk to the air quality of the environment and incidental distress to the nature of roadside plants. L. is an evergreen medicinal tree cultivated along the roadside of Lahore City. This research aimed to investigate physiological, morphological and genomorphic characteristics of under the influence of air pollution from vehicles. Healthy and mature leaves were collected from trees on Canal Bank and Mall roads of Lahore as the experimental sites and control sites were 20 km away from the experimental site. Different physiochemical, morphological, air pollution tolerance index (APTI) and molecular analysis for the detection of DNA damage were performed through comet assay. The results demonstrated the mean accumulated Cd, Pb, Cu and Ni heavy metal contents on the leaves were higher than the control plants (1.27, 3.22, 1.32 and 1.46 μg mg). APTI of trees was 9.04. Trees in these roads significantly (p < 0.01) had a lower leaf area, petiole length and leaf dry matter content in comparison to control site. Increased comet tail showed that DNA damage was higher for roadside trees than trees in the control area. For tolerance of air pollution, it necessary to check the APTI value for the at the polluted road side of Lahore city. For long-term screening, the source and type of pollutants and consistent monitoring of various responses given by the trees should be known.
汽车尾气排放出不同类型的污染物，这些污染物对环境空气质量构成极大风险，并对路边植物的生长造成附带损害。L. 是一种生长在拉合尔市路边的常绿药用树。本研究旨在调查受车辆空气污染影响下该树种的生理、形态和基因组特征。从拉合尔运河堤岸和购物中心道路的树上采集健康成熟的叶子作为实验地点，对照地点距离实验地点20公里。通过彗星试验对叶子进行了不同的理化、形态、空气污染耐受指数（APTI）和DNA损伤检测的分子分析。结果表明，叶子上镉、铅、铜和镍的平均积累重金属含量高于对照植物（分别为1.27、3.22、1.32和1.46微克/毫克）。树木的APTI为9.04。与对照地点相比，这些道路上的树木叶片面积、叶柄长度和叶片干物质含量显著较低（p < 0.01）。彗星尾的增加表明路边树木的DNA损伤高于对照区域的树木。为了评估空气污染耐受性，有必要检查拉合尔市污染路边该树种的APTI值。对于长期监测，应了解污染物的来源和类型以及树木给出的各种反应的持续监测情况。
