Lin Xiuying, Fang Yanqiu, Mi Xuguang, Fu Jianhua, Chen Shiling, Wu Mengxue, Jin Ningyi
Department of Pathology and Pathophysiology, School of Basic Medical Sciences, Yanbian University, Yanbian 133002, China.
Center of Reproductive Medicine, Jilin Province People's Hospital, Changchun 130021, China.
Regen Ther. 2024 Mar 16;27:63-72. doi: 10.1016/j.reth.2024.02.003. eCollection 2024 Dec.
The endometrium is essential to the development of embryos and pregnancy. Human umbilical cord mesenchymal stem cells (HUCMSCs) are promising stem cell sources. HUCMSCs self-renew quickly and are painless to collect. Spermidine is an inherent polyamine needed for cellular and molecular processes that regulate physiology and function. HUCMSCs and spermidine (SN) may heal intrauterine adhesions. HUCMSCs were investigated for endometrial repair in rats. Composite hydrogels are used for medical exosome implantation, including their materials, properties, and embedding procedures. This study examined whether bioengineered hydrogel-loaded exosomes from HUCMSCs and spermidine prenatally improved conception rates in mice with poor endometrial lining. The data show that HUCMSC and SN provide a good experimental base for HUCMSC safety and intrauterine treatment in rats. Western blots, exosome structural analysis, pregnancy outcomes, flow cytometry, H&E staining, immunohistochemistry, and immunofluorescence labelling found and recovered the aberrant area. HUCM-derived stem cells and spermidine-derived exosomes biophysically match. These traits strengthen and prolong endometrial function. Pregnant rats with HUCMSC and SN had thinner endometrium. Hydrogel-incorporated HEHUCMSC and SN exosomes may improve IUI in rats with thin endometrium.
子宫内膜对胚胎发育和妊娠至关重要。人脐带间充质干细胞（HUCMSCs）是很有前景的干细胞来源。HUCMSCs自我更新迅速且采集无痛。亚精胺是调节生理和功能的细胞及分子过程所需的一种内源性多胺。HUCMSCs和亚精胺（SN）可能治愈宫腔粘连。研究了HUCMSCs对大鼠子宫内膜修复的作用。复合水凝胶用于医学外泌体植入，包括其材料、特性和包埋程序。本研究检测了产前来自HUCMSCs和亚精胺的生物工程水凝胶负载外泌体是否能提高子宫内膜薄的小鼠的受孕率。数据表明，HUCMSC和SN为大鼠HUCMSC安全性和宫内治疗提供了良好的实验基础。蛋白质免疫印迹法、外泌体结构分析、妊娠结局、流式细胞术、苏木精-伊红染色、免疫组织化学和免疫荧光标记发现并修复了异常区域。HUCM来源的干细胞和亚精胺来源的外泌体在生物物理上相匹配。这些特性增强并延长了子宫内膜功能。接受HUCMSC和SN的妊娠大鼠子宫内膜较薄。掺入水凝胶的HEHUCMSC和SN外泌体可能改善子宫内膜薄的大鼠的宫腔内人工授精情况。
