Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, No. 2 Zhongguancun North First Street, Haidian District, Beijing 100190, China.
Guangdong Provincial Key Laboratory of Advanced Biomaterials, Department of Biomedical Engineering, Southern University of Science and Technology, No. 1088 Xueyuan Avenue, Nanshan District, Shenzhen, Guangdong 518055, China.
Biomacromolecules. 2024 Apr 8;25(4):2574-2586. doi: 10.1021/acs.biomac.4c00105. Epub 2024 Mar 25.
Developing biocompatible injectable hydrogels with high mechanical strength and rapid strong tissue adhesion for hemostatic sealing of uncontrolled bleeding remains a prevailing challenge. Herein, we engineer an injectable and photo-cross-linkable hydrogel based on naturally derived gelatin methacrylate (GelMA) and -hydroxysuccinimide-modified poly(γ-glutamic acid) (γPGA-NHS). The chemically dual-cross-linked hydrogel rapidly forms after UV light irradiation and covalently bonds to the underlying tissue to provide robust adhesion. We demonstrate a significantly improved hemostatic efficacy of the hydrogel using various injury models in rats compared to the commercially available fibrin glue. Notably, the hydrogel can achieve hemostasis in porcine liver and spleen incision, and femoral artery puncture models. Moreover, the hydrogel is used for sutureless repair of the liver defect in a rat model with a significantly suppressed inflammatory response, enhanced angiogenesis, and superior healing efficacy compared to fibrin glue. Together, this study offers a promising bioadhesive for treating severe bleeding and facilitating wound repair.
开发具有高强度机械性能和快速强组织黏附性的生物相容性可注射水凝胶，用于控制失控性出血的止血密封，仍然是一个亟待解决的挑战。在此，我们基于天然衍生的甲基丙烯酰化明胶（GelMA）和 - 羟基琥珀酰亚胺修饰的聚（γ-谷氨酸）（γPGA-NHS），设计了一种可注射和光交联的水凝胶。在紫外光照射后，化学双重交联的水凝胶迅速形成，并与下面的组织共价键合，提供强大的黏附力。与市售纤维蛋白胶相比，我们在大鼠的各种损伤模型中证明了该水凝胶具有显著提高的止血效果。值得注意的是，该水凝胶可实现猪肝脏和脾脏切口以及股动脉穿刺模型的止血。此外，该水凝胶用于大鼠模型中无缝合的肝脏缺陷修复，与纤维蛋白胶相比，其炎症反应显著抑制、血管生成增强、愈合效果更优。总的来说，这项研究为治疗严重出血和促进伤口修复提供了一种有前途的生物黏附剂。
