Sun Fan, Xiao Gutian, Qu Zhaoxia
University of Pittsburgh Cancer Institute, University of Pittsburgh, Pittsburgh, USA.
Department of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, USA.
Bio Protoc. 2017 May 20;7(10). doi: 10.21769/BioProtoc.2287.
A basic Bronchoalveolar lavage (BAL) procedure in mouse is described here. Cells and fluids obtained from BAL can be analyzed by Hema3-staining, immunostaining, Fluorescence-activated cell sorting (FACS), PCR, bicinchoninic acid protein assay, enzyme-linked immunosorbent assay (ELISA), luminex assays, ., to examine the immune cells, pathogens, proteins such as cytokines/chemokines, and the expression levels of inflammation-related and other genes in the cells. This will help to understand the underlying mechanisms of these lung diseases and develop specific and effective drugs.
本文描述了小鼠支气管肺泡灌洗（BAL）的基本操作流程。通过苏木精-伊红染色、免疫染色、荧光激活细胞分选（FACS）、聚合酶链反应（PCR）、二喹啉甲酸蛋白测定、酶联免疫吸附测定（ELISA）、液相芯片检测等方法，可以对BAL获得的细胞和液体进行分析，以检测免疫细胞、病原体、细胞因子/趋化因子等蛋白质以及细胞中炎症相关基因和其他基因的表达水平。这将有助于了解这些肺部疾病的潜在机制，并开发出特异性的有效药物。
