Research & Development Department, Microphyt, 713 Route de Mudaison, 34670 Baillargues, France.
Amylgen SAS, 2196 Boulevard de la Lironde, 34980 Montferrier-sur-Lez, France.
Mar Drugs. 2024 Feb 21;22(3):99. doi: 10.3390/md22030099.
The microalgae (PT) is distinguished by its rich nutrient profile, characterized by well-documented neuroprotective activities, including fucoxanthin (FX), a major carotenoid and polyunsaturated omega-3 fatty acids (n-3 PUFA). The current study aims to evaluate the protective effects of a standardized extract of PT (Mi136) containing 2% FX on cognitive function, oxidative stress, and inflammation parameters in a mouse model of accelerated aging. Seventy-two (72) male mice were randomly assigned to the blank control group (BC), negative control group (NC), and four similar microalgae extract of PT groups (branded as BrainPhyt™) with different human equivalent doses to evaluate potential dose-response effects. From day 01 to day 51, mice in the BC group were injected with a 0.9% normal saline solution, while mice in all other groups were subcutaneously injected with D-galactose (D-Gal) at a dose of 150 mg/kg once per day, five days per week. Results indicated that, for the three higher microalgae extract of PT dose groups, spatial cognitive function, swim latency, and step-through latency impairments induced by chronic D-Gal intoxication were significantly and fully inhibited, with mean values similar to those in the BC group during each day of testing. Similar benefits were observed in biochemical analysis, specifically regarding brain and plasma levels of lipid peroxidation, TNF-α, and IL-6 markers. These data underscore the positive effects of a standardized extract of PT containing 2% FX on cognitive function parameters such as spatial working memory, long-term memory, and short-term memory through the regulation of oxidative stress and inflammation pathways.
该微藻（PT）以其丰富的营养成分而闻名，具有经过充分记录的神经保护活性，包括岩藻黄素（FX），一种主要的类胡萝卜素和多不饱和ω-3 脂肪酸（n-3 PUFA）。本研究旨在评估含有 2%FX 的标准化 PT 提取物（Mi136）对加速衰老小鼠模型认知功能、氧化应激和炎症参数的保护作用。72 只雄性小鼠被随机分配到空白对照组（BC）、阴性对照组（NC）和四个类似的 PT 微藻提取物组（标记为 BrainPhyt™），每组具有不同的人类等效剂量，以评估潜在的剂量反应效应。从第 01 天到第 51 天，BC 组的小鼠注射 0.9%生理盐水溶液，而所有其他组的小鼠每天皮下注射 150mg/kg 的 D-半乳糖（D-Gal），每周五天。结果表明，对于三个较高剂量的 PT 微藻提取物组，慢性 D-Gal 中毒引起的空间认知功能、游泳潜伏期和穿梭潜伏期的损伤显著且完全被抑制，其平均值与 BC 组在测试的每一天相似。在生化分析中也观察到了类似的益处，特别是在大脑和血浆中脂质过氧化、TNF-α 和 IL-6 标志物的水平。这些数据强调了含有 2%FX 的标准化 PT 提取物对认知功能参数的积极影响，例如空间工作记忆、长期记忆和短期记忆，通过调节氧化应激和炎症途径。
