Institute of Clinical Nutrition, University of Hohenheim, Fruwirthstr. 12, 70593 Stuttgart, Germany.
Institute of Interfacial Process Engineering and Plasma Technology, University of Stuttgart, 70569 Stuttgart, Germany.
Nutrients. 2022 Jun 16;14(12):2504. doi: 10.3390/nu14122504.
Microalgae such as (PT) are a sustainable source of nutrients, especially eicosapentaenoic acid (EPA), fucoxanthin (Fx), and chrysolaminarin (Chrl), the concentrations of which can vary depending on the culture conditions. We generated three types of diets containing either an EPA- and Fx-rich (EPA/Fx) or Chrl-rich microalgae (with 5, 15, or 25% added to the diet) or an isocaloric control diet (CD). These diets were evaluated over 14 days in young C57BL/6J mice for safety and bioavailability, short-chain fatty acid (SCFA) production, and microbiome analysis. Both microalgae diets increased body weight gain dose-dependently compared to the CD. Microalgae-derived EPA was well absorbed, resulting in increased liver and fat tissue levels and a decrease in the n-6:n-3 ratio in liver tissue. Both microalgae diets increased the production of selected SCFA and decreased the Firmicutes/Bacteriodota ratio, whereas the Chrl-rich diet led to an increase in . Doses of up to 4621 mg Chrl, 920 mg EPA, and 231 mg Fx per kg body weight daily were tolerated without adverse effects. This pre-clinical study shows that PT is suitable for mouse feed, with positive effects on microbiota composition and SCFA production, suggesting beneficial effects on gut health.
微藻如（PT）是营养物质的可持续来源，特别是二十碳五烯酸（EPA）、岩藻黄质（Fx）和菊粉（Chrl），其浓度可根据培养条件而有所不同。我们生成了三种含有富含 EPA 和 Fx（EPA/Fx）或富含 Chrl（添加到饮食中的 5%、15%或 25%）的微藻的饮食，或一种等热量对照饮食（CD）。这些饮食在年轻的 C57BL/6J 小鼠中进行了为期 14 天的安全性和生物利用度、短链脂肪酸（SCFA）产生和微生物组分析评估。与 CD 相比，两种微藻饮食均能剂量依赖性地增加体重增加。微藻衍生的 EPA 被很好地吸收，导致肝脏和脂肪组织中的水平增加，以及肝脏组织中 n-6:n-3 比例降低。两种微藻饮食均增加了选定的 SCFA 的产生，并降低了厚壁菌门/拟杆菌门的比例，而富含 Chrl 的饮食则导致增加。每天每公斤体重可耐受高达 4621 毫克 Chrl、920 毫克 EPA 和 231 毫克 Fx 的剂量，而无不良反应。这项临床前研究表明，PT 适合用于小鼠饲料，对微生物群落组成和 SCFA 产生有积极影响，提示对肠道健康有益。
