Di Masi Sabrina, Manrique Rodriguez Nelson Arturo, Costa Marco, De Benedetto Giuseppe Egidio, Malitesta Cosimino
Laboratory of Analytical Chemistry, Department of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, Italy.
Laboratory of Analytical and Isotopic Mass Spectrometry, Department of Cultural Heritage, University of Salento, 73100 Lecce, Italy.
Nanomaterials (Basel). 2024 Mar 18;14(6):536. doi: 10.3390/nano14060536.
Herein, chemometric-assisted synthesis of electrochemical sensors based on electropolymerised ion-imprinted polymeric (e-IIP) films was explored. Co(II)-IIPs sensors were prepared by performing electropolymerisation procedures of polymerisation mixtures comprising varying concentrations of an electroactive o-aminophenol (o-AP) monomer and Co(II) ions, respectively, according to the Taguchi L9 experimental design, exploiting the simultaneous evaluation of other controlled parameters during electrosynthesis. Each e-IIP developed from Taguchi runs was compared with the respective non-imprinted polymer (NIP) films and fitted according to Langmuir-Freudlich isotherms. Distinctive patterns of low and high-affinity films were screened based on the qualities and properties of the developed IIPs in terms of binding kinetics (KD), imprinting factor, and the heterogeneity index of produced cavities. These results can provide a generic protocol for chemometric-assisted synthesis of e-IIPs based on poly-o-AP, providing highly stable, reproducible, and high-affinity imprinted polymeric films for monitoring purposes.
在此，探索了基于电聚合离子印迹聚合物（e-IIP）膜的化学计量学辅助合成电化学传感器的方法。根据田口L9实验设计，通过分别对包含不同浓度电活性邻氨基酚（o-AP）单体和Co（II）离子的聚合混合物进行电聚合程序，制备了Co（II）-IIPs传感器，在电合成过程中同时评估其他控制参数。将田口实验得到的每个e-IIP与相应的非印迹聚合物（NIP）膜进行比较，并根据朗缪尔-弗罗因德利希等温线进行拟合。根据所制备IIP在结合动力学（KD）、印迹因子和产生空穴的非均一性指数方面的质量和特性，筛选出低亲和力和高亲和力膜的独特模式。这些结果可为基于聚邻氨基酚的e-IIP的化学计量学辅助合成提供通用方案，为监测目的提供高度稳定、可重复且具有高亲和力的印迹聚合物膜。
