Kaningini Amani Gabriel, Motlhalamme Thobo, More Garland Kgosi, Mohale Keletso Cecilia, Maaza Malik
UNESCO-UNISA Africa Chair in Nanoscience and Nanotechnology College of Graduates Studies, University of South Africa, Muckleneuk Ridge, Pretoria, 392, South Africa.
Nanosciences African Network (NANOAFNET), iThemba LABS-National Research Foundation, 1 Old Faure Road, Somerset West 7129, PO Box 722, Somerset West, Western Cape, South Africa.
Heliyon. 2023 Apr 12;9(4):e15265. doi: 10.1016/j.heliyon.2023.e15265. eCollection 2023 Apr.
Nanoparticles produced from various metal elements including copper have been used in the treatment of infectious diseases in response to antibiotic failure due to microbial resistance. Copper is recommended for use in the production of nanoparticles largely because of its accessibility and affordability. This study aimed to synthesise copper oxide nanoparticles (CuO-NPs) using leaf extracts of and assess their antibacterial, antioxidant and cytotoxicity properties. The characterization of the obtained NPs was done through X-ray diffraction (XRD), Fourier transforms infrared spectroscopy (FTIR), Scanning electron microscopy (SEM) and Energy-dispersive spectroscopy (EDS). Our results showed that the NPs had a highly crystalline, quasi-spherical shape with an average diameter of 42 nm. Also, gram-positive bacteria and were the most susceptible to CuO-NPs with MIC values of 0.62 mg/mL and 0.16 mg/mL, respectively, as shown by the broth microdilution method. In addition, CuO-NPs demonstrated strong radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibitory activity with an IC value of 10.68 ± 0.03 μg/mL. However, the cytotoxicity activity determined by (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide) (MTT) assay revealed that the CuO-NPs were not toxic to human embryonic kidney cells (HEK 293 cells) at an LC value of 66.08 ± 0.55 μg/mL. The synthesised CuO-NPs showed high antibacterial, and antioxidant potency and less toxicity. Therefore, they could be a feasible alternative source of therapeutic agents in treating bacterial and oxidative stress-induced diseases.
包括铜在内的各种金属元素制成的纳米颗粒已被用于治疗因微生物耐药导致抗生素失效的传染病。推荐使用铜来生产纳米颗粒，主要是因为其易于获取且价格低廉。本研究旨在利用[植物名称]的叶提取物合成氧化铜纳米颗粒（CuO-NPs），并评估其抗菌、抗氧化和细胞毒性特性。通过X射线衍射（XRD）、傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）和能量色散光谱（EDS）对所得纳米颗粒进行表征。我们的结果表明，这些纳米颗粒具有高度结晶的准球形形状，平均直径为42纳米。此外，肉汤微量稀释法显示，革兰氏阳性菌[细菌名称1]和[细菌名称2]对CuO-NPs最为敏感，其最低抑菌浓度（MIC）值分别为0.62毫克/毫升和0.16毫克/毫升。此外，CuO-NPs表现出较强的2,2-二苯基-1-苦基肼（DPPH）自由基抑制活性，半数抑制浓度（IC）值为10.68±0.03微克/毫升。然而，通过（3-(4,5-二甲基噻唑-2-基)-2,5-二苯基-2H-四唑溴盐）（MTT）法测定的细胞毒性活性表明，在半数致死浓度（LC）值为66.08±0.55微克/毫升时，CuO-NPs对人胚肾细胞（HEK 293细胞）无毒。合成的CuO-NPs具有较高的抗菌和抗氧化能力，且毒性较小。因此，它们可能是治疗细菌和氧化应激诱导疾病的可行替代治疗剂来源。
