Department of Molecular and Computational Biology, University of Southern California, Los Angeles, CA, United States of America.
Department of Ecology and Evolutionary Biology, University of California Santa Cruz, Santa Cruz, CA, United States of America.
PLoS One. 2024 Mar 27;19(3):e0295740. doi: 10.1371/journal.pone.0295740. eCollection 2024.
Microbial inoculants can increase the yield of cultivated crops and are successful in independent trials; however, efficacy drops in large-scale applications due to insufficient consideration of microbial community dynamics. The structure of microbiomes, in addition to the impact of individual taxa, is an important factor to consider when designing growth-promoting inoculants. Here, we investigate the microbial network and community assembly patterns of Macrocystis pyrifera gametophyte germplasm cultures (collectively referred to as a "seedbank") used to cultivate an offshore farm in Santa Barbara, California, and identify network features associated with increased biomass of mature sporophytes. We found that [1] several network features, such as clustering coefficient and edge ratios, significantly vary with biomass outcomes; [2] gametophytes that become low- or high-biomass sporophytes have different hub taxa; and [3] microbial community assembly of gametophyte germplasm cultures is niche-driven. Overall, this study describes microbial community dynamics in M. pyrifera germplasm cultures and ultimately supports the development of early life stage inoculants that can be used on seaweed cultivars to increase biomass yield.
微生物接种剂可以提高栽培作物的产量，并且在独立试验中取得了成功；然而，由于对微生物群落动态的考虑不足，其在大规模应用中的效果会下降。在设计促进生长的接种剂时，除了个别分类群的影响外，微生物组的结构也是一个重要的考虑因素。在这里，我们研究了加利福尼亚州圣巴巴拉近海养殖场使用的大型巨藻配子体种质培养物（统称为“种子库”）的微生物网络和群落组装模式，并确定了与成熟孢子体生物量增加相关的网络特征。我们发现：[1] 几个网络特征，如聚类系数和边比，与生物量结果显著相关；[2] 成为低生物量或高生物量孢子体的配子体具有不同的枢纽分类群；[3] 配子体种质培养物的微生物群落组装是由生态位驱动的。总的来说，本研究描述了大型巨藻种质培养物中的微生物群落动态，并最终支持了早期生命阶段接种剂的开发，这些接种剂可以用于海藻品种以增加生物量产量。
