Department of Chemical Engineering, SUNY College of Environmental Science and Forestry, Syracuse, NY 13210, United States.
Department of Chemical Engineering, SUNY College of Environmental Science and Forestry, Syracuse, NY 13210, United States.
Biotechnol Adv. 2024 May-Jun;72:108340. doi: 10.1016/j.biotechadv.2024.108340. Epub 2024 Mar 26.
As an energy-storage substance of microorganisms, polyhydroxybutyrate (PHB) is a promising alternative to petrochemical polymers. Under appropriate fermentation conditions, PHB-producing strains with metabolic diversity can efficiently synthesize PHB using various carbon sources. Carbon-rich wastes may serve as alternatives to pure sugar substrates to reduce the cost of PHB production. Genetic engineering strategies can further improve the efficiency of substrate assimilation and PHB synthesis. In the downstream link, PHB recycling strategies based on green chemistry concepts can replace PHB extraction using chlorinated solvents to enhance the economics of PHB production and reduce the potential risks of environmental pollution and health damage. To avoid carbon loss caused by biodegradation in the traditional sense, various strategies have been developed to degrade PHB waste into monomers. These monomers can serve as platform chemicals to synthesize other functional compounds or as substrates for PHB reproduction. The sustainable potential and cycling value of PHB are thus reflected. This review summarized the recent progress of strains, substrates, and fermentation approaches for microbial PHB production. Analyses of available strategies for sustainable PHB recycling were also included. Furthermore, it discussed feasible pathways for PHB waste valorization. These contents may provide insights for constructing PHB-based comprehensive biorefinery systems.
聚羟基丁酸酯（PHB）作为微生物的储能物质，是一种有前途的石化聚合物替代品。在适当的发酵条件下，具有代谢多样性的 PHB 产生菌可以使用各种碳源高效地合成 PHB。富碳废物可以替代纯糖底物，以降低 PHB 生产的成本。遗传工程策略可以进一步提高基质同化和 PHB 合成的效率。在下游环节，基于绿色化学概念的 PHB 回收策略可以替代使用氯化溶剂提取 PHB，从而提高 PHB 生产的经济性，降低环境污染和健康损害的潜在风险。为了避免传统意义上生物降解造成的碳损失，已经开发出各种策略来将 PHB 废物降解为单体。这些单体可以用作合成其他功能化合物的平台化学品，也可以作为 PHB 再生产的底物。因此，PHB 具有可持续潜力和循环价值。本综述总结了微生物 PHB 生产中菌株、底物和发酵方法的最新进展。还分析了可持续 PHB 回收的现有策略。此外，还讨论了 PHB 废物增值的可行途径。这些内容可能为构建基于 PHB 的综合生物炼制系统提供思路。
