Zhu Yanan, Shao Changyan, Martín-Orúe Susana María
School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.
Animal Nutrition and Welfare Service, Animal and Food Science Department, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
Foods. 2024 Mar 21;13(6):952. doi: 10.3390/foods13060952.
Enterotoxigenic (ETEC) is the major diarrhoea-causing pathogen world-wide. Fimbria-receptor recognition is the primary step when attachment of ETEC to the intestine occurs. This study aims to evaluate the potential of some traditional foods, particularly those rich in β-glucans, as analogues for fimbriae or receptors in reducing ETEC colonisation. The adhesion test (AT) demonstrated that aqueous extracts of highland barley (EHB), black rice (EBR) and little millet (ELT) at concentrations of 2% and 1% could attach to more ETEC K88ac ( < 0.001), as well as aqueous extracts of shiitake (EST) ( < 0.01). The competition test (CT) revealed that EHB and EST significantly prevented ETEC K88ac from adhering to intestinal epithelial cells (IPEC-J2) at 2% ( < 0.01) and 1% ( < 0.05). In the Exclusion Test (ET) and the displacement test (DT), the food samples were unable to impair ETEC colonisation in terms of blocking receptors or removing attached pathogens. These results demonstrate how some traditional foods such as highland barley and shiitake contain bioactive compounds that interfere with the attachment of ETEC to the intestinal epithelium, and their potential in the prevention and treatment of ETEC diarrhoea.
产肠毒素大肠杆菌（ETEC）是全球主要的致腹泻病原体。菌毛-受体识别是ETEC附着于肠道时的首要步骤。本研究旨在评估一些传统食物，特别是富含β-葡聚糖的食物，作为菌毛或受体类似物在减少ETEC定植方面的潜力。黏附试验（AT）表明，浓度为2%和1%的青稞水提取物（EHB）、黑米水提取物（EBR）和粟米水提取物（ELT）能附着更多的ETEC K88ac（<0.001），香菇水提取物（EST）也是如此（<0.01）。竞争试验（CT）显示，EHB和EST在浓度为2%（<0.01）和1%（<0.05）时能显著阻止ETEC K88ac黏附于肠上皮细胞（IPEC-J2）。在排除试验（ET）和置换试验（DT）中，食物样本在阻断受体或清除附着病原体方面无法削弱ETEC定植。这些结果表明，青稞和香菇等一些传统食物含有生物活性化合物，可干扰ETEC附着于肠上皮，以及它们在预防和治疗ETEC腹泻方面的潜力。
