Forest and Fruit Tree Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
Shanghai Key Labs of Protected Horticultural Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
Int J Mol Sci. 2024 Mar 12;25(6):3221. doi: 10.3390/ijms25063221.
Resistance (R) genes were used to recognize pathogen effectors directly or indirectly in plants and activate defense signal pathways. Most of these R proteins consist of a nucleotide-binding adaptor (NB-ARC) domain, a leucine-rich repeat (LRR) domain and some also have a coiled-coil (CC) structure. In this study, we cloned a gene which encodes the CC-NB-ARC-LRR R protein () from Chinese wild grapevine accession 'Dan-2'. The transcript of was obviously induced by inoculation with and the salicylic acid (SA) treatment. The results of sequence analysis showed that the gene contained a CC domain at the N-terminus, along with an NB-ARC and an LRR domain at the C-terminus. We transferred this gene into wildtype and treated transgenic lines with () and pv. DC3000 ( DC3000); the results demonstrated that promotes broad spectrum resistance to pathogens. Furthermore, qPCR analysis displayed that may display a significant function in disease resistance via activating SA signaling pathways. In general, these conclusions primarily demonstrated that enhances the disease resistance level in plants and contributes to future research of the gene identification for grape breeding biotechnology.
抗性（R）基因被用于直接或间接地识别植物中的病原体效应物，并激活防御信号通路。这些 R 蛋白大多数由核苷酸结合适配器（NB-ARC）结构域、富含亮氨酸重复（LRR）结构域组成，有些还具有卷曲螺旋（CC）结构。本研究从中国野生葡萄品种‘Dan-2’中克隆了一个基因，该基因编码 CC-NB-ARC-LRR R 蛋白（）。的转录本明显受到接种和水杨酸（SA）处理的诱导。序列分析结果表明，基因在 N 端含有 CC 结构域，在 C 端含有 NB-ARC 和 LRR 结构域。我们将该基因转入野生型和处理过的转基因系中（）和 pv. DC3000（ DC3000）；结果表明，促进了对病原体的广谱抗性。此外，qPCR 分析表明，可能通过激活 SA 信号通路在抗病性中发挥重要作用。总的来说，这些结论主要表明，增强了植物的抗病水平，为葡萄育种生物技术中该基因的鉴定研究做出了贡献。
