State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi, China.
Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, China.
Plant Biotechnol J. 2018 Aug;16(8):1488-1501. doi: 10.1111/pbi.12891. Epub 2018 Mar 8.
As one of the most serious diseases in grape, downy mildew caused by Plasmopara viticola is a worldwide grape disease. Much effort has been focused on improving susceptible grapevine resistance, and wild resistant grapevine species are important for germplasm improvement of commercial cultivars. Using yeast two-hybrid screen followed by a series of immunoprecipitation experiments, we identified voltage-dependent anion channel 3 (VDAC3) protein from Vitis piasezkii 'Liuba-8' as an interacting partner of VpPR10.1 cloned from Vitis pseudoreticulata 'Baihe-35-1', which is an important germplasm for its resistance to a range of pathogens. Co-expression of VpPR10.1/VpVDAC3 induced cell death in Nicotiana benthamiana, which accompanied by ROS accumulation. VpPR10.1 transgenic grapevine line showed resistance to P. viticola. We conclude that the VpPR10.1/VpVDAC3 complex is responsible for cell death-mediated defence response to P. viticola in grapevine.
作为葡萄上最严重的病害之一，由葡萄霜霉病菌引起的霜霉病是一种世界性的葡萄病害。人们一直致力于提高易感葡萄的抗性，而野生抗性葡萄种对于商业品种的种质改良非常重要。利用酵母双杂交筛选，随后进行一系列免疫沉淀实验，我们从葡萄品种‘琉璃巴八’中鉴定出电压依赖性阴离子通道 3（VDAC3）蛋白是从葡萄品种‘白河-35-1’克隆的 VpPR10.1 的互作蛋白，而‘白河-35-1’是一种对多种病原体具有抗性的重要种质资源。VpPR10.1/VpVDAC3 的共表达在本氏烟中诱导细胞死亡，同时伴随着 ROS 的积累。VpPR10.1 转基因葡萄品系对葡萄霜霉病菌表现出抗性。我们的结论是，VpPR10.1/VpVDAC3 复合物是葡萄对葡萄霜霉病菌引起的细胞死亡介导的防御反应的关键。
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