NADPH 氧化酶在乳腺癌中的基因表达及预后价值。

Gene Expression and Prognostic Value of NADPH Oxidase Enzymes in Breast Cancer.

机构信息

Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-971, Brazil.

出版信息

Int J Mol Sci. 2024 Mar 19;25(6):3464. doi: 10.3390/ijms25063464.

DOI:10.3390/ijms25063464

PMID:38542437

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10970871/

Abstract

NADPH oxidase enzymes (NOX) are involved in all stages of carcinogenesis, but their expression levels and prognostic value in breast cancer (BC) remain unclear. Thus, we aimed to assess the expression and prognostic value of NOX enzymes in BC samples using online databases. For this, mRNA expression from 290 normal breast tissue samples and 1904 BC samples obtained from studies on cBioPortal, Kaplan-Meier Plotter, and The Human Protein Atlas were analyzed. We found higher levels of NOX2, NOX4, and Dual oxidase 1 (DUOX1) in normal breast tissue. NOX1, NOX2, and NOX4 exhibited higher expression in BC, except for the basal subtype, where NOX4 expression was lower. DUOX1 mRNA levels were lower in all BC subtypes. NOX2, NOX4, and NOX5 mRNA levels increased with tumor progression stages, while NOX1 and DUOX1 expression decreased in more advanced stages. Moreover, patients with low expression of NOX1, NOX4, and DUOX1 had lower survival rates than those with high expression of these enzymes. In conclusion, our data suggest an overexpression of NOX enzymes in breast cancer, with certain isoforms showing a positive correlation with tumor progression.

摘要

NADPH 氧化酶（NOX）参与了癌症发生的所有阶段，但它们在乳腺癌（BC）中的表达水平和预后价值仍不清楚。因此，我们旨在使用在线数据库评估 BC 样本中 NOX 酶的表达和预后价值。为此，我们分析了来自 cBioPortal、Kaplan-Meier Plotter 和 The Human Protein Atlas 的 290 份正常乳腺组织样本和 1904 份 BC 样本的 mRNA 表达。我们发现正常乳腺组织中 NOX2、NOX4 和双氧化酶 1（DUOX1）的水平较高。NOX1、NOX2 和 NOX4 在 BC 中表达较高，但基底亚型除外，NOX4 表达较低。所有 BC 亚型的 DUOX1mRNA 水平均较低。NOX2、NOX4 和 NOX5mRNA 水平随肿瘤进展阶段而升高，而 NOX1 和 DUOX1 的表达在更晚期阶段下降。此外，NOX1、NOX4 和 DUOX1 低表达的患者的生存率低于这些酶高表达的患者。总之，我们的数据表明，NOX 酶在乳腺癌中过度表达，某些同工酶与肿瘤进展呈正相关。