College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China.
The United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima 890-0065, Japan.
Molecules. 2024 Mar 16;29(6):1329. doi: 10.3390/molecules29061329.
In Asian regions, areca nuts are tropical fruits that are extensively consumed. The areca nut contains a lot of polyphenols and its safety is unknown. In this research, we investigated the effects of lipopolysaccharides (LPS) and areca nut polyphenols (ANP) on normal RAW264.7 cells. The results showed that LPS stimulated adverse effects in normal cells by affecting cytokine production. The GO analysis results mainly affected DNA repair, cell division, and enzyme activities. In the KEGG analysis results, the NOD-like receptor signaling pathway, which is related to NF-κB, MAPK, and the pro-inflammatory cytokines, is the most significant. In the protein-protein interaction network (PPI) results, significant sub-networks in all three groups were shown to be related to cytokine-cytokine receptor interaction. Collectively, our findings showed a comprehensive understanding of LPS-induced toxicity and the protective effects of ANP by RNA sequencing.
在亚洲地区，槟榔是一种热带水果，被广泛食用。槟榔含有大量的多酚，其安全性未知。在这项研究中，我们研究了脂多糖（LPS）和槟榔多酚（ANP）对正常 RAW264.7 细胞的影响。结果表明，LPS 通过影响细胞因子的产生对正常细胞产生不良影响。GO 分析结果主要影响 DNA 修复、细胞分裂和酶活性。在 KEGG 分析结果中，与 NF-κB、MAPK 和促炎细胞因子相关的 NOD 样受体信号通路最为显著。在蛋白质-蛋白质相互作用网络（PPI）结果中，三组的显著子网络均与细胞因子-细胞因子受体相互作用有关。综上所述，我们的研究结果通过 RNA 测序全面了解了 LPS 诱导的毒性和 ANP 的保护作用。
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