Martín Cristina, Ferreiro Fernández Adalyz, Salazar Romero Julia C, Fernández-Blázquez Juan P, Mendizabal Jabier, Artola Koldo, Jorcano José L, Rabanal M Eugenia
Department of Bioengineering, Universidad Carlos III de Madrid, 28911 Leganés, Spain.
Department of Materials Science and Engineering and Chemical Engineering & IAAB, Universidad Carlos III de Madrid, 28911 Leganés, Spain.
Pharmaceutics. 2024 Feb 22;16(3):305. doi: 10.3390/pharmaceutics16030305.
In recent years, the rapid emergence of antibiotic-resistant bacteria has become a significant concern in the healthcare field, and although bactericidal dressings loaded with various classes of antibiotics have been used in clinics, in addition to other anti-infective strategies, this alarming issue necessitates the development of innovative strategies to combat bacterial infections and promote wound healing. Electrospinning technology has gained significant attention as a versatile method for fabricating advanced wound dressings with enhanced functionalities. This work is based on the generation of polyvinylpyrrolidone (PVP)-based dressings through electrospinning, using a DomoBIO4A bioprinter, and incorporating graphene oxide (GO)/zinc oxide (ZnO) nanocomposites as a potent antibacterial agent. GO and ZnO nanoparticles offer unique properties, including broad-spectrum antibacterial activity for improved wound healing capabilities. The synthesis process was performed in an inexpensive one-pot reaction, and the nanocomposites were thoroughly characterized using XRD, TEM, EDX, SEM, EDS, and TGA. The antibacterial activity of the dispersions was demonstrated against and , Gram-negative and Gram-positive bacteria, respectively, using the well diffusion method and the spread plate method. Bactericidal mats were synthesized in a rapid and cost-effective manner, and the fiber-based structure of the electrospun dressings was studied by SEM. Evaluations of their antibacterial efficacy against and were explored by the disk-diffusion method, revealing an outstanding antibacterial capacity, especially against the Gram-positive strain. Overall, the findings of this research contribute to the development of next-generation wound dressings that effectively combat bacterial infections and pave the way for advanced therapeutic interventions in the field of wound care.
近年来，抗生素耐药菌的迅速出现已成为医疗保健领域的一个重大问题。尽管临床上已使用负载各类抗生素的杀菌敷料以及其他抗感染策略，但这一令人担忧的问题仍需要开发创新策略来对抗细菌感染并促进伤口愈合。静电纺丝技术作为一种制备具有增强功能的先进伤口敷料的通用方法，已受到广泛关注。这项工作基于使用DomoBIO4A生物打印机通过静电纺丝生成基于聚乙烯吡咯烷酮（PVP）的敷料，并将氧化石墨烯（GO）/氧化锌（ZnO）纳米复合材料作为一种有效的抗菌剂纳入其中。GO和ZnO纳米颗粒具有独特的性能，包括具有广谱抗菌活性以提高伤口愈合能力。合成过程采用廉价的一锅法反应进行，并且使用XRD、TEM、EDX、SEM、EDS和TGA对纳米复合材料进行了全面表征。分别使用平板扩散法和涂布平板法证明了分散体对革兰氏阴性菌和革兰氏阳性菌的抗菌活性。以快速且经济高效的方式合成了杀菌垫，并通过SEM研究了静电纺丝敷料的纤维基结构。通过纸片扩散法探索了它们对革兰氏阴性菌和革兰氏阳性菌的抗菌效果评估，结果显示出出色的抗菌能力，尤其是对革兰氏阳性菌株。总体而言，本研究的结果有助于开发下一代有效对抗细菌感染的伤口敷料，并为伤口护理领域的先进治疗干预铺平道路。
