Martín Cristina, Bachiller Ariadna, Fernández-Blázquez Juan P, Nishina Yuta, Jorcano José L
Department of Bioengineering, Universidad Carlos III de Madrid, Leganés 28911, Spain.
Institute IMDEA Materials, Getafe 28906, Spain.
ACS Mater Lett. 2023 Mar 23;5(4):1245-1255. doi: 10.1021/acsmaterialslett.2c01044. eCollection 2023 Apr 3.
Wound infection is inevitable in most patients suffering from extensive burns or chronic ulcers, and there is an urgent demand for the production of bactericidal dressings to be used as grafts to restore skin functionalities. In this context, the present study explores the fabrication of plasma-derived fibrin hydrogels containing bactericidal hybrids based on graphene oxide (GO). The hydrogels were fully characterized regarding gelation kinetics, mechanical properties, and internal hydrogel structures by disruptive cryo scanning electron microscopies (cryo-SEMs). The gelation kinetic experiments revealed an acceleration of the gel formation when GO was added to the hydrogels in a concentration of up to 0.2 mg/mL. The cryo-SEM studies showed up a decrease of the pore size when GO was added to the network, which agreed with a faster area contraction and a higher compression modulus of the hydrogels that contained GO, pointing out the critical structural role of the nanomaterial. Afterward, to study the bactericidal ability of the gels, GO was used as a carrier, loading streptomycin (STREP) on its surface. The loading content of the drug to form the hybrid (GO/STREP) resulted in 50.2% ± 4.7%, and the presence of the antibiotic was also demonstrated by Raman spectroscopy, Z-potential studies, and thermogravimetric analyses. The fibrin-derived hydrogels containing GO/STREP showed a dose-response behavior according to the bactericidal hybrid concentration and allowed a sustained release of the antibiotic at a programmed rate, leading to drug delivery over a prolonged period of time.
对于大多数大面积烧伤或患有慢性溃疡的患者来说，伤口感染是不可避免的，因此迫切需要生产用作移植物以恢复皮肤功能的杀菌敷料。在此背景下，本研究探索了基于氧化石墨烯（GO）制备含杀菌杂化物的血浆源性纤维蛋白水凝胶。通过破坏性冷冻扫描电子显微镜（cryo-SEM）对水凝胶的凝胶化动力学、力学性能和内部水凝胶结构进行了全面表征。凝胶化动力学实验表明，当向水凝胶中添加浓度高达0.2 mg/mL的GO时，凝胶形成加速。cryo-SEM研究显示，当向网络中添加GO时，孔径减小，这与含GO的水凝胶更快的面积收缩和更高的压缩模量一致，指出了纳米材料的关键结构作用。之后，为了研究凝胶的杀菌能力，将GO用作载体，在其表面负载链霉素（STREP）。形成杂化物（GO/STREP）的药物负载量为50.2%±4.7%，拉曼光谱、Z电位研究和热重分析也证实了抗生素的存在。含GO/STREP的纤维蛋白衍生水凝胶根据杀菌杂化物浓度表现出剂量反应行为，并允许抗生素以设定速率持续释放，从而实现长时间的药物递送。
