Animal and Veterinary Research Centre (CECAV), AL4AnimaLS, Department of Veterinary Sciences, School of Agricultural and Veterinary Sciences, University of Trás-os-Montes e Alto Douro (UTAD), Vila Real, Portugal.
VetLamaçães, Veterinary Clinic, Braga, Portugal.
Vet Med Sci. 2024 May;10(3):e1236. doi: 10.1002/vms3.1236.
The increasing interest in platelet-based therapies has underwritten the development of novel veterinary regenerative treatments. The haemoderivative platelet-rich fibrin (PRF) comprises abundant concentrations of platelets and leucocytes, above the physiologic baseline, which are considered essential elements for wound regeneration, stimulating local angiogenesis, cellular migration, proliferation and differentiation, considered essential for skin repair.
This study aimed to describe the treatment of eight dogs with naturally occurring cutaneous wounds, where autologous PRF therapy was applied, using a protocol developed by our group.
Eight dogs, aged between 7-month and 9-year old, from different breeds and sexes, were enrolled in this study. Four of these wounds were clinically infected. In three cases, two PRF treatments were performed during the first week of treatment, followed by single weekly treatments from the second week onwards, until exophytic granulation tissues were present. In each case, the treatment was finalized only when complete wound closure was achieved. Wounds did not receive topical antiseptics, antibiotics or topical drugs to promote wound healing during the treatment.
PRF-grafting treatments were well tolerated in all treated wounds, inducing significant granulation tissue formation. PRF clots acted as a natural tissue filler, promoting epithelization and wound closure, without the requirement of topical antimicrobial/antiseptics application, or additional surgical debridement. Evident skin contraction was recorded in larger injuries and all the treatments resulted in vestigial aesthetic scars where hair growth was also observed.
PRF-therapy obtained promising results, as an alternative wound treatment, revealing a biological regenerative action, prompting the natural skin healing process.
对基于血小板的治疗方法的兴趣日益浓厚，促成了新型兽医再生治疗方法的发展。血液衍生的富含血小板纤维蛋白（PRF）包含大量的血小板和白细胞，超过生理基线，这些被认为是伤口再生所必需的元素，刺激局部血管生成、细胞迁移、增殖和分化，这被认为对皮肤修复至关重要。
本研究旨在描述使用我们小组开发的方案对 8 只患有自然发生的皮肤伤口的狗进行自体 PRF 治疗的情况。
本研究纳入了 8 只不同品种和性别的狗，年龄在 7 个月至 9 岁之间。其中 4 只伤口有临床感染。在 3 例中，在治疗的第一周内进行了两次 PRF 治疗，然后从第二周开始每周进行一次治疗，直到出现外生性肉芽组织。在每种情况下，只有当完全闭合伤口时才完成治疗。在治疗过程中，伤口未使用局部防腐剂、抗生素或促进伤口愈合的局部药物。
PRF 移植治疗在所有接受治疗的伤口中均耐受良好，诱导了明显的肉芽组织形成。PRF 凝块充当天然组织填充剂，促进上皮化和伤口闭合，无需应用局部抗菌/防腐剂，也无需额外的手术清创。较大的伤口记录到明显的皮肤收缩，所有治疗均导致残留的美容疤痕，同时也观察到毛发再生。
PRF 治疗作为一种替代伤口治疗方法取得了有希望的结果，显示出生物再生作用，促进了自然皮肤愈合过程。
