解析流感保护相关性：来自人类 A/H1N1 挑战的经验教训。
Unravelling influenza correlates of protection: lessons from human A/H1N1 Challenge.
机构信息
Department of Clinical Science, The Influenza Centre, University of Bergen, Bergen, Norway.
Department of Microbiology, Haukeland University Hopsital, Bergen, Norway.
出版信息
mBio. 2024 May 8;15(5):e0006424. doi: 10.1128/mbio.00064-24. Epub 2024 Mar 28.
Abstract
Mucosal immunity is important in protecting from upper respiratory tract influenza infection. Human challenge provides a unique model to define correlates of protection with baseline immune responses being correlated to the quantity and length of viral shedding and clinical outcomes. Here, we discuss recent work on mucosal and systemic correlates of protection (R. Bean, L. T. Giurgea, A. Han, L. Czajkowski, et al., mBio 15:e02372-23, 2024, https://doi.org/10.1128/mbio.02372-23) and place it in the context of previous work on mucosal immunity. We also discuss the importance of standardized assays to allow global comparison of relevant immune responses in defining correlates of protection. Correlates of protection are important for designing next-generation broadly protective influenza vaccines.
摘要
黏膜免疫对于预防上呼吸道流感感染非常重要。人类挑战模型为确定保护相关因素提供了一个独特的模型，基础免疫反应与病毒脱落的数量和时间以及临床结果相关。在这里，我们讨论了黏膜和系统保护相关因素的最新研究进展（R. Bean、L. T. Giurgea、A. Han、L. Czajkowski 等人，mBio 15:e02372-23, 2024, https://doi.org/10.1128/mBio.02372-23），并将其置于以前关于黏膜免疫的研究背景下进行讨论。我们还讨论了标准化检测方法的重要性，这对于在确定保护相关因素时，对相关免疫反应进行全球比较非常重要。保护相关因素对于设计新一代广泛保护性流感疫苗非常重要。
相似文献
1
Unravelling influenza correlates of protection: lessons from human A/H1N1 Challenge.解析流感保护相关性：来自人类 A/H1N1 挑战的经验教训。
mBio. 2024 May 8;15(5):e0006424. doi: 10.1128/mbio.00064-24. Epub 2024 Mar 28.
2
Meeting report and review: Immunological assays and correlates of protection for next-generation influenza vaccines.会议报告和综述：下一代流感疫苗的免疫测定和保护相关性。
Influenza Other Respir Viruses. 2020 Mar;14(2):237-243. doi: 10.1111/irv.12706. Epub 2019 Dec 13.
3
Broadly protective immunity against divergent influenza viruses by oral co-administration of Lactococcus lactis expressing nucleoprotein adjuvanted with cholera toxin B subunit in mice.在小鼠中通过口服共同给予表达核蛋白的乳酸乳球菌并佐以霍乱毒素B亚基，对多种流感病毒产生广泛的保护性免疫。
Microb Cell Fact. 2015 Aug 5;14:111. doi: 10.1186/s12934-015-0287-4.
4
Evaluation of Antihemagglutinin and Antineuraminidase Antibodies as Correlates of Protection in an Influenza A/H1N1 Virus Healthy Human Challenge Model.在甲型H1N1流感病毒健康人体激发模型中评估抗血凝素和抗神经氨酸酶抗体作为保护相关性指标的研究
mBio. 2016 Apr 19;7(2):e00417-16. doi: 10.1128/mBio.00417-16.
5
Activation and Induction of Antigen-Specific T Follicular Helper Cells Play a Critical Role in Live-Attenuated Influenza Vaccine-Induced Human Mucosal Anti-influenza Antibody Response.抗原特异性滤泡辅助 T 细胞的激活和诱导在活流感减毒疫苗诱导的人体黏膜抗流感抗体反应中发挥关键作用。
J Virol. 2018 May 14;92(11). doi: 10.1128/JVI.00114-18. Print 2018 Jun 1.
6
Single-dose mucosal immunization with a candidate universal influenza vaccine provides rapid protection from virulent H5N1, H3N2 and H1N1 viruses.单剂量黏膜免疫接种候选通用流感疫苗可快速预防高致病性 H5N1、H3N2 和 H1N1 病毒。
PLoS One. 2010 Oct 4;5(10):e13162. doi: 10.1371/journal.pone.0013162.
7
Human influenza virus challenge identifies cellular correlates of protection for oral vaccination.人类流感病毒挑战鉴定口服疫苗保护的细胞相关性。
Cell Host Microbe. 2021 Dec 8;29(12):1828-1837.e5. doi: 10.1016/j.chom.2021.10.009. Epub 2021 Nov 15.
8
Neuraminidase-Inhibiting Antibody Titers Correlate with Protection from Heterologous Influenza Virus Strains of the Same Neuraminidase Subtype.神经氨酸酶抑制抗体滴度与对同一神经氨酸酶亚型的异源流感病毒株的保护作用相关。
J Virol. 2018 Aug 16;92(17). doi: 10.1128/JVI.01006-18. Print 2018 Sep 1.
9
Inactivated Influenza Vaccine That Provides Rapid, Innate-Immune-System-Mediated Protection and Subsequent Long-Term Adaptive Immunity.一种能提供快速的、由先天免疫系统介导的保护以及后续长期适应性免疫的灭活流感疫苗。
mBio. 2015 Oct 27;6(6):e01024-15. doi: 10.1128/mBio.01024-15.
10
Modified vaccinia virus Ankara expressing the hemagglutinin of pandemic (H1N1) 2009 virus induces cross-protective immunity against Eurasian 'avian-like' H1N1 swine viruses in mice.改良安卡拉痘苗病毒表达的大流行（H1N1）2009 病毒血凝素诱导小鼠对欧亚“禽源样”H1N1 猪病毒产生交叉保护免疫。
Influenza Other Respir Viruses. 2014 May;8(3):367-75. doi: 10.1111/irv.12221. Epub 2013 Dec 23.
引用本文的文献
1
Binding antibody titers against the hemagglutinin and neuraminidase correlate with protection against medically attended influenza A and B disease.针对血凝素和神经氨酸酶的结合抗体滴度与预防甲型和乙型流感就医疾病的保护作用相关。
J Virol. 2025 Jun 17;99(6):e0039125. doi: 10.1128/jvi.00391-25. Epub 2025 May 13.
本文引用的文献
1
Mucosal correlates of protection after influenza viral challenge of vaccinated and unvaccinated healthy volunteers.接种疫苗和未接种疫苗的健康志愿者接种流感病毒后黏膜相关性保护。
mBio. 2024 Feb 14;15(2):e0237223. doi: 10.1128/mbio.02372-23. Epub 2024 Jan 9.
2
Proficiency tests to evaluate the impact on assay outcomes of harmonized influenza-specific Intracellular Cytokine Staining (ICS) and IFN-ɣ Enzyme-Linked ImmunoSpot (ELISpot) protocols.能力验证试验，以评估统一的流感特异性细胞内细胞因子染色（ICS）和干扰素-γ酶联免疫斑点法（ELISpot）方案对检测结果的影响。
J Immunol Methods. 2023 Dec;523:113584. doi: 10.1016/j.jim.2023.113584. Epub 2023 Oct 31.
3
Haemagglutination inhibition and virus microneutralisation serology assays: use of harmonised protocols and biological standards in seasonal influenza serology testing and their impact on inter-laboratory variation and assay correlation: A FLUCOP collaborative study.血凝抑制和病毒微量中和血清学检测：在季节性流感血清学检测中使用协调一致的方案和生物标准及其对实验室间变异和检测相关性的影响：FLUCOP 合作研究。
Front Immunol. 2023 Apr 18;14:1155552. doi: 10.3389/fimmu.2023.1155552. eCollection 2023.
4
Harmonization and qualification of intracellular cytokine staining to measure influenza-specific CD4 T cell immunity within the FLUCOP consortium.协调并鉴定细胞内细胞因子染色法以测量流感特异性 CD4 T 细胞免疫应答：FLUCOP 联盟内的研究
Front Immunol. 2022 Oct 20;13:982887. doi: 10.3389/fimmu.2022.982887. eCollection 2022.
5
Harmonization and qualification of an IFN-γ Enzyme-Linked ImmunoSpot assay (ELISPOT) to measure influenza-specific cell-mediated immunity within the FLUCOP consortium.协调和鉴定一种 IFN-γ 酶联免疫斑点（ELISPOT）检测方法，以在 FLUCOP 联盟内测量流感特异性细胞介导免疫。
Front Immunol. 2022 Sep 8;13:984642. doi: 10.3389/fimmu.2022.984642. eCollection 2022.
6
SARS-CoV-2 vaccination induces mucosal antibody responses in previously infected individuals.SARS-CoV-2 疫苗接种可诱导既往感染者产生黏膜抗体应答。
Nat Commun. 2022 Sep 1;13(1):5135. doi: 10.1038/s41467-022-32389-8.
7
Validation of a Harmonized Enzyme-Linked-Lectin-Assay (ELLA-NI) Based Neuraminidase Inhibition Assay Standard Operating Procedure (SOP) for Quantification of N1 Influenza Antibodies and the Use of a Calibrator to Improve the Reproducibility of the ELLA-NI With Reverse Genetics Viral and Recombinant Neuraminidase Antigens: A FLUCOP Collaborative Study.验证一种基于酶联凝集素检测（ELLA-NI）的神经氨酸酶抑制检测标准操作程序（SOP），用于定量 N1 型流感抗体，并使用校准品提高基于反向遗传病毒和重组神经氨酸酶抗原的 ELLA-NI 的重现性：FLUCOP 协作研究。
Front Immunol. 2022 Jun 17;13:909297. doi: 10.3389/fimmu.2022.909297. eCollection 2022.
8
Assay Harmonization and Use of Biological Standards To Improve the Reproducibility of the Hemagglutination Inhibition Assay: a FLUCOP Collaborative Study.测定均一化和生物标准品的使用以提高血凝抑制试验的重现性：FLUCOP 协作研究。
mSphere. 2021 Aug 25;6(4):e0056721. doi: 10.1128/mSphere.00567-21. Epub 2021 Jul 28.
9
Meeting report and review: Immunological assays and correlates of protection for next-generation influenza vaccines.会议报告和综述：下一代流感疫苗的免疫测定和保护相关性。
Influenza Other Respir Viruses. 2020 Mar;14(2):237-243. doi: 10.1111/irv.12706. Epub 2019 Dec 13.
10
Live-Attenuated Influenza Vaccine Induces Tonsillar Follicular T Helper Cell Responses That Correlate With Antibody Induction.减毒流感疫苗诱导扁桃体滤泡辅助性 T 细胞应答，与抗体诱导相关。
J Infect Dis. 2020 Jan 1;221(1):21-32. doi: 10.1093/infdis/jiz321.
Zotero 插件