General Sciences Department, Faculty of Education, Soran University, Soran, Erbil Kurdistan Region of Iraq.
JBRA Assist Reprod. 2024 Aug 26;28(3):382-391. doi: 10.5935/1518-0557.20240010.
Clinacanthus nutans (C. nutans) is a medicinal herb that most people with diabetes have historically taken. It's a diet high in antioxidants, which are supposed to help people live longer and be healthier. It is the first study to suggest using C. nutans to enhance the quality of sperm in male mice given a streptozotocin (STZ) injection.
Sixty mice were divided into two groups at the age of four weeks: group one was fed a regular diet (n=10), while group two was administered a high-fat diet (n=50) for eight weeks to develop obesity. Obese mice were given 100mg/kg of STZ to produce hyperglycemia with a 20% mortality rate. Then, 40 hyperglycemic mice were separated into two groups: STZ (n=10) and sample (n=30). The treatment groups were administered a methanolic extract of C. nutans leaves by gavage at doses of 150, 300, and 500mg/kg of body weight (n=10) for 4 weeks.
In contrast to the STZ group, there was a substantial (p=0.001) drop in serum blood glucose and total sperm abnormalities in mice at varying doses. Catalase, glutathione s-transferase (GST), and total antioxidant capacity significantly (p=0.001) increased in the STZ mice group at varying doses, but malondialdehyde was reduced. In comparison to STZ mice, testosterone and luteinizing hormone (LH) levels improved in mice treated with extracts of C. nutans at various doses. For all of the following dependent variables, extraction of the leaf at higher concentrations of 500 milligrams/kilogram has better efficacy than 300 and 150 mg/kg after 4 weeks of treatment.
The research and development of new natural agents to combat oxidative stress-related diseases have sparked a lot of interest. As a result, the potential leaf extract of C. nutans contains anti-hyperglycemic compounds and improves the quality of sperm in male mice.
灵香草（C. nutans）是一种药用草本植物，历史上大多数糖尿病患者都在服用。它的饮食富含抗氧化剂，据称可以帮助人们活得更久、更健康。这是第一项研究表明，使用灵香草可以提高给予链脲佐菌素（STZ）注射的雄性小鼠的精子质量。
60 只小鼠在四周大时分为两组：一组喂食常规饮食（n=10），另一组喂食高脂肪饮食（n=50）八周以发展肥胖。肥胖小鼠给予 100mg/kg 的 STZ 以产生高血糖，死亡率为 20%。然后，将 40 只高血糖小鼠分为两组：STZ（n=10）和样品（n=30）。治疗组通过灌胃给予灵香草叶甲醇提取物，剂量分别为 150、300 和 500mg/kg 体重（n=10），持续 4 周。
与 STZ 组相比，不同剂量下小鼠的血清血糖和总精子异常明显下降（p=0.001）。不同剂量下 STZ 组小鼠的过氧化氢酶、谷胱甘肽 S-转移酶（GST）和总抗氧化能力显著增加（p=0.001），而丙二醛减少。与 STZ 组相比，用灵香草提取物治疗的小鼠的睾酮和促黄体生成激素（LH）水平在不同剂量下均有所改善。对于所有以下依赖变量，在经过 4 周的治疗后，浓度为 500 毫克/千克的叶片提取物的提取效果优于 300 和 150 毫克/千克。
开发新的天然药物来对抗与氧化应激相关的疾病引起了广泛关注。因此，灵香草的潜在叶提取物含有抗高血糖化合物，可以提高雄性小鼠的精子质量。
