Li Maiquan, Dong Yunxia, Kang Mangjun, Tao Tiantian, Li Wenlan, Zhang Sheng, Quan Wei, Liu Zhonghua
College of Food Science and Technology, Hunan Provincial Key Laboratory of Food Science and Biotechnology, Hunan Agricultural University, Changsha 410128, China.
Key Laboratory of Tea Science of Ministry of Education, National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, College of Horticulture, Hunan Agricultural University, Changsha 410128, China.
Food Chem X. 2024 Mar 16;22:101296. doi: 10.1016/j.fochx.2024.101296. eCollection 2024 Jun 30.
Hyperglycemia can cause early damage to human bady and develop into diabates that will severely threaten human healthy. The effectively clinical treatment of hyperglycemiais is by inhibiting the activity of α-amylase. Black tea has been reported to show inhibitory effect on α-amylase and can be used for hyperglycemia treatment. However, the mechanism underlying is unclear. In this study, experiment showed that black tea theaflavins extract (BTE) effectively alleviated hyperglycemia. experiment showed that the effects may be caused by the interation between theaflavins and α-amylase. While TF1 and TF3 were mixed type inhibitors of α-amylase, TF2A and TF2B were competitive inhibitors of α-amylase. Molecular docking analysis showed that theaflavins monomers interacted with the hydrophobic region of α-amylase. Further study verified that monomer-α-amylase complex was spontaneously formed depending on hydrophobic interactions. Taken together, theaflavins showed potential anti-hyperglycemia effect via inhibiting α-amylase activity. Our results suggested that theaflavins might be utilized as a new type of α-amylase inhibitor to prevent and cure hyperglycemia.
高血糖会对人体造成早期损害，并发展成糖尿病，严重威胁人类健康。高血糖的有效临床治疗方法是抑制α-淀粉酶的活性。据报道，红茶对α-淀粉酶有抑制作用，可用于治疗高血糖。然而，其潜在机制尚不清楚。在本研究中，实验表明红茶茶黄素提取物（BTE）能有效缓解高血糖。实验表明，这种作用可能是由茶黄素与α-淀粉酶之间的相互作用引起的。虽然TF1和TF3是α-淀粉酶的混合型抑制剂，但TF2A和TF2B是α-淀粉酶的竞争性抑制剂。分子对接分析表明，茶黄素单体与α-淀粉酶的疏水区域相互作用。进一步研究证实，单体-α-淀粉酶复合物是通过疏水相互作用自发形成的。综上所述，茶黄素通过抑制α-淀粉酶活性显示出潜在的抗高血糖作用。我们的研究结果表明，茶黄素可能作为一种新型的α-淀粉酶抑制剂用于预防和治疗高血糖。
