Botelho Letícia Pena, Dias De Oliveira Simone Gomes, Douglas De Oliveira Dhelfeson Willya, Galo Rodrigo
Department of Dentistry, School of Biological and Health Sciences, Federal University of Jequitinhonha and Mucuri Valley, Diamantina, Brazil.
Department of Dental Materials and Prosthesis, School of Dentistry, University of São Paulo, Ribeirão Preto, Brazil.
J Conserv Dent. 2022 May-Jun;25(3):278-282. doi: 10.4103/jcd.jcd_612_21. Epub 2022 Jun 13.
The aim of this study was to evaluate whether 2.5% chitosan applied after dentin acid etching or incorporated into the adhesive system interferes with the microtensile strength of the interface tooth-resin.
Sixty healthy bovine teeth had the incisal thirds and the roots sectioned and were randomly allocated into three groups ( = 60). G1 (control): acid attack + Clearfil SE Bond + Z350 resin; G2: treatment with 2.5% chitosan + Clearfil SE Bond + Z350 resin; G3: treatment with 2.5% chitosan incorporated into dentin adhesive + Z350 resin. The stick-shaped specimens made in each group were randomly divided into two subgroups and subjected to degradation of the adhesive interface (with aging: specimens immersed in 2.5% sodium hypochlorite for 12 h and washed in distilled water for 1 h, and without aging). The groups were submitted to a microtensile strength test with a 0.5 mm/min speed until rupture. Statistical analysis of the data was performed according to normal distribution.
Microtensile data were obtained by calculating the area of each toothpick, obtaining values in megapascal. No aging: G1 - 5251.69, G2 - 5.52 ± 1.46, and G3 - 3.91 ± 1.21. With aging: G1 - 3.45 ± 1.29, G2 - 2.75 ± 0.78, and G3 - 3.53 ± 1.33. Statistical analysis showed that the aging factor and adhesive modification decreased the bonding strength of the resin-dentin ( = 0.001). As well, the interaction between the analyzed factors showed statistically significant differences ( = 0.002).
Accelerated aging decreases the adhesion strength in G1, even when chitosan is previously applied to the adhesive system. However, when adding chitosan to the adhesive, adhesion remained similar after aging but lower when compared to the other groups.
本研究旨在评估在牙本质酸蚀后应用2.5%的壳聚糖或将其加入黏结系统是否会干扰牙-树脂界面的微拉伸强度。
60颗健康牛牙将切端三分之一和牙根进行切片，并随机分为三组（n = 60）。G1（对照组）：酸蚀+Clearfil SE Bond黏结剂+Z350树脂；G2：用2.5%壳聚糖处理+Clearfil SE Bond黏结剂+Z350树脂；G3：用加入牙本质黏结剂中的2.5%壳聚糖处理+Z350树脂。每组制作的棒状试件随机分为两个亚组，并进行黏结界面的降解处理（老化：试件浸泡于2.5%次氯酸钠中12小时，然后用蒸馏水冲洗1小时；未老化）。各组以0.5毫米/分钟的速度进行微拉伸强度测试直至断裂。根据正态分布对数据进行统计分析。
通过计算每根牙签的面积获得微拉伸数据，单位为兆帕。未老化：G1 - 5251.69，G2 - 5.52±1.4,6，G3 - 3.91±1.21。老化后：G1 - 3.45±1.29，G2 - 2.75±0.78，G3 - 3.53±1.33。统计分析表明，老化因素和黏结剂改性降低了树脂-牙本质的黏结强度（P = 0.001）。同样，分析因素之间的相互作用显示出统计学上的显著差异（P = 0.002）。
加速老化会降低G1组中的黏附强度，即使壳聚糖预先应用于黏结系统。然而，当在黏结剂中添加壳聚糖时，老化后黏附力保持相似，但与其他组相比更低。
