Freiman J, Eckstein R, McCaughan G, Parsons C, Davies J S, Diegutis P, Burnett L, Gallagher N
Hepatology. 1985 Jan-Feb;5(1):50-3. doi: 10.1002/hep.1840050112.
The correlation between serum and hepatic markers of hepatitis B virus (HBV) has been studied in 70 subjects with chronic active hepatitis of whom 18 were HBsAg+ and 52 were HBsAg-. In HBsAg+ subjects, sera were tested for HBeAg/anti-HBe status and for HBV DNA sequences using a DNA dot hybridization technique. Anti-HBs and anti-HBc were measured in serum in the HBsAg- group. Immunoperoxidase staining was used to detect HBsAg, HBcAg and delta antigen in liver tissue. Of the 18 HBsAg+ patients, 13 were HBeAg+ and 5 were anti-HBe+. A good correlation was shown between HBeAg and HBV DNA in serum and HBcAg expression in liver tissue. Neither HBV DNA in serum nor HBcAg in liver tissue was detected in any of the anti-HBe+ patients. HBsAg and/or HBcAg were detected in liver tissue in 17 of 18 HBsAg+ subjects (95%). However, neither HBsAg nor HBcAg were detected in liver tissue in 52 HBsAg- patients. This group included 11 patients with antibody markers in serum of past HBV infection. Thus, in contrast to previous studies, a good correlation was demonstrated between the serum and hepatic markers of viral replication, and no evidence was obtained to implicate the HBV as an etiological agent in HBsAg- chronic active hepatitis.
对70例慢性活动性肝炎患者进行了乙肝病毒（HBV）血清标志物与肝脏标志物之间相关性的研究，其中18例HBsAg阳性，52例HBsAg阴性。在HBsAg阳性患者中，采用DNA斑点杂交技术检测血清中的HBeAg/抗-HBe状态及HBV DNA序列。在HBsAg阴性组中检测血清中的抗-HBs和抗-HBc。采用免疫过氧化物酶染色法检测肝组织中的HBsAg、HBcAg和δ抗原。18例HBsAg阳性患者中，13例HBeAg阳性，5例抗-HBe阳性。血清中的HBeAg与HBV DNA以及肝组织中的HBcAg表达之间显示出良好的相关性。在任何抗-HBe阳性患者中均未检测到血清中的HBV DNA或肝组织中的HBcAg。18例HBsAg阳性患者中有17例（95%）肝组织中检测到HBsAg和/或HBcAg。然而，52例HBsAg阴性患者的肝组织中均未检测到HBsAg和HBcAg。该组包括11例血清中有既往HBV感染抗体标志物的患者。因此，与以往研究不同，本研究证明了病毒复制的血清标志物与肝脏标志物之间具有良好的相关性，且未获得证据表明HBV是HBsAg阴性慢性活动性肝炎的病因。
