PPR-DYW Protein EMP17 Is Required for Mitochondrial RNA Editing, Complex III Biogenesis, and Seed Development in Maize.

The conversion of cytidines to uridines (C-to-U) at specific sites in mitochondrial and plastid transcripts is a post-transcriptional processing event that is important to the expression of organellar genes. Pentatricopeptide repeat (PPR) proteins are involved in this process. In this study, we report the function of a previously uncharacterized PPR-DYW protein, Empty pericarp17 (EMP17), in the C-to-U editing and kernel development in maize. EMP17 is targeted to mitochondria. The loss-function of EMP17 arrests maize kernel development, abolishes the editing at -799 and -677 sites, and reduces the editing at -906 and -966 sites. The absence of editing causes amino acid residue changes in CcmF-267 (Ser to Pro) and Nad2-226 (Phe to Ser), respectively. As CcmF functions in cytochrome (Cyt) maturation, the amount of Cyt and Cyt protein is drastically reduced in , suggesting that the CcmF-267 (Ser to Pro) change impairs the CcmF function. As a result, the assembly of complex III is strikingly decreased in . In contrast, the assembly of complex I appears less affected, suggesting that the Nad2-226 (Phe to Ser) change may have less impact on Nad2 function. Together, these results indicate that EMP17 is required for the C-to-U editing at several sites in mitochondrial transcripts, complex III biogenesis, and seed development in maize.