Zhang Meina, Dop Romy A, Zhang Haifei
Department of Chemistry, University of Liverpool, Crown Street, Liverpool L69 7ZD, U.K.
Department of Clinical Infection, Microbiology and Immunology, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool L69 7ZD, U.K.
ACS Omega. 2024 Mar 13;9(12):14465-14474. doi: 10.1021/acsomega.4c00263. eCollection 2024 Mar 26.
Nanofibrous polymeric materials, combined with protein therapeutics, play a significant role in biomedical and pharmaceutical applications. However, the upload of proteins into nanofibers with a high yield and controlled release has been a challenging issue. Here, we report the in situ loading of a model protein (bovine serum albumin) into hydrophilic poly(vinyl alcohol) nanofibers via ice-templating, with a 100% protein drug loading efficiency. These protein-loaded nanofibers were further coated by polydopamine in order to improve the nanofiber stability and achieve a controlled protein release. The mass ratio between poly(vinyl alcohol) and bovine serum albumin influenced the percentage of proteins in composite nanofibers and fiber morphology. More particles and less nanofibers were formed with an increasing percentage of bovine serum albumin. By varying the coating conditions, it was possible to produce a uniform polydopamine coating with tunable thickness, which acted as an additional barrier to reduce burst release and achieve a more sustained release profile.
纳米纤维聚合物材料与蛋白质疗法相结合，在生物医学和制药应用中发挥着重要作用。然而，将蛋白质高产率且可控地负载到纳米纤维中一直是一个具有挑战性的问题。在此，我们报道了通过冰模板法将一种模型蛋白质（牛血清白蛋白）原位负载到亲水性聚乙烯醇纳米纤维中，蛋白质药物负载效率达100%。这些负载蛋白质的纳米纤维进一步用聚多巴胺包覆，以提高纳米纤维的稳定性并实现蛋白质的可控释放。聚乙烯醇与牛血清白蛋白之间的质量比影响复合纳米纤维中蛋白质的百分比和纤维形态。随着牛血清白蛋白百分比的增加，形成的颗粒增多，纳米纤维减少。通过改变包覆条件，可以制备出具有可调厚度的均匀聚多巴胺涂层，该涂层可作为额外的屏障来减少突释并实现更持久的释放曲线。
