Roe R, Robins R A, Laxton R R, Baldwin R W
Mol Immunol. 1985 Jan;22(1):11-21. doi: 10.1016/0161-5890(85)90029-x.
Flow cytofluorimetric methods have been used to quantitate the interaction between a divalent monoclonal antibody and a tumour cell surface antigen. After standardization using fluorescein and 125I-labelled antibodies, kinetics of association and dissociation were measured, and antibody bound at equilibrium quantitated. A mathematical model was developed in conjunction with these experimental results which allowed the calculation of rates for monovalent association and monovalent and divalent dissociation, and a description of the contribution of each to the level of bound antibody at different antibody concns.
流式细胞荧光测定法已被用于定量二价单克隆抗体与肿瘤细胞表面抗原之间的相互作用。在用荧光素和¹²⁵I标记的抗体进行标准化后，测定结合和解离动力学，并对平衡时结合的抗体进行定量。结合这些实验结果建立了一个数学模型，该模型可以计算单价结合、单价和解离的速率，并描述每种速率对不同抗体浓度下结合抗体水平的贡献。
