有丝分裂的Pt K2上皮细胞中细胞内游离钙水平降低。
Intracellular free calcium levels are reduced in mitotic Pt K2 epithelial cells.
作者信息
Keith C H, Maxfield F R, Shelanski M L
出版信息
Proc Natl Acad Sci U S A. 1985 Feb;82(3):800-4. doi: 10.1073/pnas.82.3.800.
Using a fluorescence ratio method, we have studied the intracellular free calcium levels in individual quin-2-loaded mitotic cells under the microscope. We have found that intracellular free calcium concentrations in Pt K2 epithelial cells drop by approximately 50% as they pass through mitosis. Calcium levels in interphase cells were 53 +/- 7 nM. During prophase, free cytoplasmic calcium begins to decrease, reaching 28 +/- 3 nM in prometaphase. Calcium levels remain low until the nuclear envelope is re-formed in late telophase, when they increase again to interphase levels. This decrease in overall free calcium in mitosis suggests that the mitotic cell has mechanisms for the general sequestration, and perhaps local release, of calcium ions.
我们使用荧光比率法，在显微镜下研究了单个负载喹啉-2的有丝分裂细胞内的游离钙水平。我们发现，Pt K2上皮细胞在经历有丝分裂时，细胞内游离钙浓度大约下降了50%。间期细胞中的钙水平为53±7 nM。在前期，游离细胞质钙开始减少，在前中期降至28±3 nM。钙水平一直保持较低，直到末期后期核膜重新形成时，才再次升高至间期水平。有丝分裂过程中总体游离钙的这种减少表明，有丝分裂细胞具有钙离子的一般隔离机制，或许还有局部释放机制。
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