Markiewicz L, Schatz F, Barg P, Gurpide E
J Steroid Biochem. 1985 Feb;22(2):231-5. doi: 10.1016/0022-4731(85)90117-7.
Specimens of proliferative and secretory human endometrium were incubated under organ culture or superfusion conditions and the levels of PGF2 alpha in the medium were measured by radioimmunoassay. Basal rates of PGF2 alpha output during short-term superfusions and long-term (1-2 day) batch incubations, performed on the same tissue specimens, were similar. Basal output of PGF2 alpha by proliferative endometrium (230-280 ng/mg protein X d) was significantly higher than that of secretory tissue under both experimental conditions. Estradiol (10(-8) M) increased PGF2 alpha output significantly (4-fold) only in secretory endometrium under organ culture conditions; Progesterone (10(-7) M) decreased it significantly (to 1/2-1/4 of the basal level) in both types of endometria during long-term incubations and in proliferative endometrium during superfusion. Glands isolated from proliferative and secretory endometrium produced PGF2 alpha during superfusion at a rate comparable to that of endometrial tissue under similar conditions. PGF2 alpha output by glands isolated from secretory endometrium increased significantly (3-fold) when estradiol was added to the superfusion medium.
将增殖期和分泌期的人子宫内膜标本在器官培养或灌流条件下进行孵育，并用放射免疫分析法测定培养基中前列腺素F2α（PGF2α）的水平。对相同组织标本进行短期灌流和长期（1 - 2天）批量孵育时，PGF2α的基础分泌率相似。在两种实验条件下，增殖期子宫内膜的PGF2α基础分泌量（230 - 280 ng/mg蛋白质×天）显著高于分泌期组织。仅在器官培养条件下，雌二醇（10⁻⁸ M）能使分泌期子宫内膜的PGF2α分泌量显著增加（4倍）；在长期孵育过程中，孕酮（10⁻⁷ M）能使两种类型子宫内膜的PGF2α分泌量显著降低（至基础水平的1/2 - 1/4），在灌流过程中能使增殖期子宫内膜的PGF2α分泌量显著降低。在灌流过程中，从增殖期和分泌期子宫内膜分离出的腺体产生PGF2α的速率与相似条件下的子宫内膜组织相当。当向灌流培养基中添加雌二醇时，从分泌期子宫内膜分离出的腺体的PGF2α分泌量显著增加（3倍）。
