College of Resources and Environment, Yunnan Agricultural University, Kunming, China.
Plant Cell Rep. 2024 Apr 2;43(4):109. doi: 10.1007/s00299-024-03188-w.
The regulatory action of BXs secreted by wheat on the pathogenicity of FOF causing Fusarium wilt in faba bean were analyzed. DIMBOA and MBOA weakened the pathogenicity of FOF. A large number of pathogenic bacteria in continuous cropping soil infect faba bean plants, leading to the occurrence of wilt disease, which restricts their production. Faba bean-wheat intercropping is often used to alleviate this disease. This study investigates the effect of benzoxazinoids (BXs) secreted by wheat root on the pathogenicity of Fusarium oxysporum f. sp. Fabae (FOF) and underlying molecular mechanisms. The effects of DIMBOA(2,4-dihydroxy-7-methoxy-1,4-benzoxazine-4-one) and MBOA(6-methoxybenzoxazolin-2-one) on the activity of cell-wall-degrading enzymes in FOF(cellulase, pectinase, amylase, and protease), FOF Toxin (fusaric acid, FA) content were investigated through indoor culture experiments. The effect of BXs on the metabolic level of FOF was analyzed by metabonomics to explore the ecological function of benzoxazines intercropping control of Fusarium wilt in faba bean. The results show that the Exogenous addition of DIMBOA and MBOA decreased the activity of plant-cell-wall-degrading enzymes and fusaric acid content and significantly weakened the pathogenicity of FOF. DIMBOA and MBOA significantly inhibited the pathogenicity of FOF, and metabolome analysis showed that DIMBOA and MBOA reduced the pathogenicity of FOF by down-regulating related pathways such as nucleotide metabolism and linoleic acid metabolism, thus effectively controlling the occurrence of Fusarium wilt in faba bean.
分析了小麦分泌的 BXs 对引起蚕豆枯萎病的 FOF 致病性的调节作用。DIMBOA 和 MBOA 削弱了 FOF 的致病性。连作土壤中的大量病原菌感染蚕豆植株，导致枯萎病的发生，从而限制了其产量。蚕豆-小麦间作常被用来缓解这种病害。本研究探讨了小麦根系分泌的苯并恶嗪类化合物（BXs）对尖孢镰刀菌（FOF）及其致病机制的影响。通过室内培养实验，研究了 DIMBOA（2,4-二羟基-7-甲氧基-1,4-苯并恶嗪-4-酮）和 MBOA（6-甲氧基苯并恶唑啉-2-酮）对 FOF 细胞壁降解酶（纤维素酶、果胶酶、淀粉酶和蛋白酶）活性的影响，FOF 毒素（镰刀菌酸，FA）含量。通过代谢组学分析 BXs 对 FOF 代谢水平的影响，探讨苯并恶嗪类化合物间作对蚕豆枯萎病的生态控制功能。结果表明，外源添加 DIMBOA 和 MBOA 降低了植物细胞壁降解酶和镰刀菌酸含量的活性，显著削弱了 FOF 的致病性。DIMBOA 和 MBOA 显著抑制 FOF 的致病性，代谢组学分析表明，DIMBOA 和 MBOA 通过下调核苷酸代谢和亚油酸代谢等相关途径降低 FOF 的致病性，从而有效控制蚕豆枯萎病的发生。
