Zheng Yiran, Guo Yuting, Lv Jiaxing, Dong Kun, Dong Yan
College of Resources and Environment, Yunnan Agricultural University, Kunming650000, China.
College of Animal Science and Technology, Yunnan Agricultural University, Kunming650000, China.
ACS Omega. 2023 Jan 10;8(3):2897-2906. doi: 10.1021/acsomega.2c04569. eCollection 2023 Jan 24.
Continuous cropping leads to the accumulation of autotoxic substances in faba beans, which limits their global production. Intercropping is widely used to alleviate these problems. This study aims to explore the important role of f. sp. (FOF) and benzoic acid stress in enhancing the occurrence of faba bean Fusarium wilt and the potential mechanism of faba bean-wheat intercropping to control the occurrence of this disease. We analyzed the pathogenic mechanism of FOF and benzoic acid and the defense response of faba bean-wheat intercropping against the autotoxicity of benzoic acid under hydroponic conditions that included the pathogen alone and in combination with different concentrations of benzoic acid. The dual stress of FOF and benzoic acid inhibited the activity of defensive enzymes, the synthesis of defensive substances, and the expression of defensive genes in faba bean roots and reduced the disease resistance of faba bean. This shows that benzoic acid plays an important role in helping FOF cause disease. Faba bean-wheat intercropping improves plant resistance by alleviating benzoic acid stress and reducing the incidence and disease index of Fusarium wilt. The dual stress of FOF and benzoic acid promotes the occurrence of faba bean Fusarium wilt by destroying the root defense system of faba bean. Faba bean-wheat intercropping can effectively alleviate the autotoxicity of benzoic acid and control the occurrence of Fusarium wilt by improving the physiological and biochemical resistance of faba beans and the expression of defense genes.
连作导致蚕豆中自毒物质积累，限制了其全球产量。间作被广泛用于缓解这些问题。本研究旨在探讨尖孢镰刀菌蚕豆专化型（FOF）和苯甲酸胁迫在加剧蚕豆枯萎病发生中的重要作用，以及蚕豆-小麦间作控制该病发生的潜在机制。我们在水培条件下分析了FOF和苯甲酸的致病机制以及蚕豆-小麦间作对苯甲酸自毒作用的防御反应，水培条件包括单独接种病原菌以及与不同浓度苯甲酸组合接种。FOF和苯甲酸的双重胁迫抑制了蚕豆根中防御酶活性、防御物质合成及防御基因表达，降低了蚕豆的抗病性。这表明苯甲酸在协助FOF致病中起重要作用。蚕豆-小麦间作通过减轻苯甲酸胁迫、降低枯萎病发病率和病情指数来提高植株抗性。FOF和苯甲酸的双重胁迫通过破坏蚕豆根系防御系统促进蚕豆枯萎病发生。蚕豆-小麦间作可有效减轻苯甲酸自毒作用，并通过提高蚕豆生理生化抗性和防御基因表达来控制枯萎病发生。
