College of Resources and Environment, Yunnan Agricultural University, Kunming, China.
Plant Mol Biol. 2024 Apr 17;114(3):42. doi: 10.1007/s11103-024-01439-8.
Continuous cropping of faba bean (Vicia faba L.) has led to a high incidence of wilt disease. The implementation of an intercropping system involving wheat and faba bean can effectively control the propagation of faba bean wilt disease. To investigate the mechanisms of wheat in mitigating faba bean wilt disease in a wheat-faba bean intercropping system. A comprehensive investigation was conducted to assess the temporal variations in Fusarium oxysporum f. sp. fabae (FOF) on the chemotaxis of benzoxazinoids (BXs) and wheat root through indoor culture tests. The effects of BXs on FOF mycelial growth, spore germination, spore production, and electrical conductivity were examined. The influence of BXs on the ultrastructure of FOF was investigated through transmission electron microscopy. Eukaryotic mRNA sequencing was utilized to analyze the differentially expressed genes in FOF upon treatment with BXs. FOF exhibited a significant positive chemotactic effect on BXs in wheat roots and root secretions. BXs possessed the potential to exert significant allelopathic effects on the mycelial growth, spore germination, and sporulation of FOF. In addition, BXs demonstrated a remarkable ability to disrupt the structural integrity and stability of the membrane and cell wall of the FOF mycelia. BXs possessed the capability of posing threats to the integrity and stability of the cell membrane and cell wall. This ultimately resulted in physiological dysfunction, effectively inhibiting the regular growth and developmental processes of the FOF.
连作菘蓝（Vicia faba L.）导致萎蔫病发病率高。实施小麦与菘蓝间作系统可以有效控制菘蓝枯萎病的传播。为了研究小麦在小麦-菘蓝间作系统中减轻菘蓝枯萎病的机制。通过室内培养试验，全面调查了禾谷镰刀菌（FOF）对苯并恶嗪类化合物（BXs）和小麦根趋化性的时间变化。研究了 BXs 对 FOF 菌丝生长、孢子萌发、孢子产生和电导率的影响。通过透射电子显微镜研究了 BXs 对 FOF 超微结构的影响。利用真核 mRNA 测序分析了 BXs 处理后 FOF 中差异表达的基因。FOF 对小麦根和根分泌物中的 BXs 表现出显著的正趋化作用。BXs 对 FOF 的菌丝生长、孢子萌发和孢子产生具有显著的化感作用。此外，BXs 表现出破坏 FOF 菌丝细胞膜和细胞壁结构完整性和稳定性的显著能力。BXs 具有威胁细胞膜和细胞壁完整性和稳定性的能力。这最终导致生理功能障碍，有效抑制了 FOF 的正常生长和发育过程。
