Watanabe T, Sariban E, Mitchell T, Kufe D
Biochem Biophys Res Commun. 1985 Feb 15;126(3):999-1005. doi: 10.1016/0006-291x(85)90284-0.
The genome of the human HL-60 promyelocytic leukemia cell contains amplified c-myc sequences and the transforming N-ras oncogene. The present study has monitored c-myc and N-ras expression in HL-60 cells during induction of myeloid and monocytic differentiation with dimethyl sulfoxide, hexamethylene bisacetamide, 12-O-tetradecanoylphorbol-13-acetate and 1,25 dihydroxy-vitamin D3. The results demonstrate that induction of HL-60 differentiation is associated with decreases in c-myc RNA, while there is little if any effect on expression of the N-ras gene. Although the diminution in c-myc expression occurred as an early event in the induction of HL-60 differentiation, the rate of decrease in c-myc transcripts varied with respect to cessation of proliferation. Thus, the appearance of the mature phenotype and loss of proliferative capacity are associated with declines in c-myc RNA, while these events appear to occur in the absence of significant alterations in N-ras expression.
人类HL-60早幼粒细胞白血病细胞的基因组含有扩增的c-myc序列和具有转化作用的N-ras癌基因。本研究监测了用二甲基亚砜、六亚甲基双乙酰胺、12-O-十四酰佛波醇-13-乙酸酯和1,25-二羟基维生素D3诱导HL-60细胞进行髓系和单核细胞分化过程中c-myc和N-ras的表达。结果表明，HL-60细胞分化的诱导与c-myc RNA的减少有关，而对N-ras基因的表达几乎没有影响。虽然c-myc表达的减少是HL-60细胞分化诱导过程中的早期事件，但c-myc转录本的减少速率相对于增殖停止而言有所不同。因此，成熟表型的出现和增殖能力的丧失与c-myc RNA的下降有关，而这些事件似乎在N-ras表达没有明显改变的情况下发生。
