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恩诺沙星纸片扩散法药敏试验的拟议质量控制及解释标准

Proposed quality control and interpretive criteria for disk diffusion susceptibility testing with enoxacin.

作者信息

Rudrik J T, Cavalieri S J, Britt E M

出版信息

J Clin Microbiol. 1985 Mar;21(3):332-4. doi: 10.1128/jcm.21.3.332-334.1985.

Abstract

The standardized disk diffusion test, in which a 10-micrograms enoxacin disk is used, was performed and microbroth dilution MICs were determined to establish individual test control values with Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, and S. aureus ATCC 29213. In addition, regression analysis correlating inhibitory zone diameter with MICs for approximately 400 gram-negative clinical isolates was performed. Based on linear regression and error rate-bounded analyses, criteria for the category calls of isolates are proposed.

摘要

使用含10微克依诺沙星的纸片进行标准化纸片扩散试验,并测定微量肉汤稀释法的最低抑菌浓度(MIC),以建立针对大肠埃希菌ATCC 25922、铜绿假单胞菌ATCC 27853、金黄色葡萄球菌ATCC 25923和金黄色葡萄球菌ATCC 29213的个体试验对照值。此外,还对约400株革兰氏阴性临床分离株的抑菌圈直径与MIC进行了回归分析。基于线性回归和误差率受限分析,提出了分离株分类判定的标准。

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